Abstract

The Fluorescence Microscopy Core provides technical expertise in fluorescence microscopy techniques and quantitative image processing applications, including optical sectioning and multi-dimensional imaging, nonlinear optical microscopy (aka 2-photon imaging), fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF) and quantitative live-cell fluorescence imaging. Services provided by the core include access to and technical assistance with state of the art instrumentation and software, and expertise in experimental design and data interpretation for fluorescence imaging applications. The core is located at the Richard D. Berlin Center for Cell Analysis and Modeling (CCAM; www.ccam.uchc.edu), and is situated on the same floor as the Center for Vascular Biology. The close proximity between CCAM and the Center for Vascular Biology makes for easy and extensive interactions between CCAM and the program group.

Public Health Relevance

The Fluorescence Microscopy Core will assist the PPG investigators with sophisticated experiments that use fluorescence microscopy and related techniques, The aim of the experiments are to visualize cells at very high magnification so that the disease process of atherosclerosis is better understood.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL070694-09
Application #
8379846
Study Section
Special Emphasis Panel (ZHL1-PPG-Y)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
9
Fiscal Year
2012
Total Cost
$99,341
Indirect Cost
$38,534

  Institution

Name
University of Connecticut
Department
Type
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030

  Publications

Gerber, Claire; Harel, Miriam; Lynch, Miranda L et al. (2016) Proximal tubule proteins are significantly elevated in bladder urine of patients with ureteropelvic junction obstruction and may represent novel biomarkers: A pilot study. J Pediatr Urol 12:120.e1-7
Harel, Miriam; Ferrer, Fernando A; Shapiro, Linda H et al. (2016) Future directions in risk stratification and therapy for advanced pediatric genitourinary rhabdomyosarcoma. Urol Oncol 34:103-15
Conway, Rebecca E; Rojas, Camilo; Alt, Jesse et al. (2016) Prostate-specific membrane antigen (PSMA)-mediated laminin proteolysis generates a pro-angiogenic peptide. Angiogenesis 19:487-500
Galvani, Sylvain; Sanson, Marie; Blaho, Victoria A et al. (2015) HDL-bound sphingosine 1-phosphate acts as a biased agonist for the endothelial cell receptor S1P1 to limit vascular inflammation. Sci Signal 8:ra79
Ghosh, Mallika; Subramani, Jaganathan; Rahman, M Mamunur et al. (2015) CD13 restricts TLR4 endocytic signal transduction in inflammation. J Immunol 194:4466-76
Blaho, Victoria A; Galvani, Sylvain; Engelbrecht, Eric et al. (2015) HDL-bound sphingosine-1-phosphate restrains lymphopoiesis and neuroinflammation. Nature 523:342-6
Ghosh, Mallika; Gerber, Claire; Rahman, M Mamunur et al. (2014) Molecular mechanisms regulating CD13-mediated adhesion. Immunology 142:636-47
Blaho, Victoria A; Hla, Timothy (2014) An update on the biology of sphingosine 1-phosphate receptors. J Lipid Res 55:1596-608
Rahman, M Mamunur; Ghosh, Mallika; Subramani, Jaganathan et al. (2014) CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury. Stem Cells 32:1564-77
Xiong, Yuquan; Lee, Hyuek Jong; Mariko, Boubacar et al. (2013) Sphingosine kinases are not required for inflammatory responses in macrophages. J Biol Chem 288:32563-73

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