The molecular genetics and proteomics core serves as a resource to provide expertise and assistance in the analysis of gene and protein expression to projects by Van Eyk and Tomaselli. The emphasis of the core will be on the preparation and utilization of mRNA and protein for the performance and analysis of microarray and protein expression studies. Activities of the core facility will be performed in several locations on the campus of the Johns Hopkins School of Medicine including laboratories in the Division of Cardiology, the Sidney Kimmel Comprehensive Cancer Care Microarray Facility and the Center for Computational Medicine and Biology centered in the Department of Biomedical Engineering. The services provided by this Core include: mRNA preparation and qualification for kinetic real time PCR (RT-PCR) and microarray experiments; access to canine EST libraries; spotting and hybridization to microarrays, assistance with analysis of microarray data; access to CAGE, a database repository for microarray experiments; primers for RT-PCR; canine tissue qualified antibodies and protocols for their use in Western blotting, immunohistochemical and immunocytochemical applications; cytosolic, sarcolemmal, sarcoplasmic reticulum, ERK1/2 and connexin subproteome preparation and analysis (including the performance of 2D gel electrophoresis (2DE), 2D liquid chromatography (2DLC), differential imaging gel electrophoresis (DICE) and mass spectrometry); and a canine heart-specific protein database for peptide mass fingerprinting (PMF). In addition to the provision of technical expertise to the projects, the core will generate significant cost savings by serving as a centralized resource for molecular biological and protein chemical analysis of the canine heart.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL077180-05
Application #
7688517
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
5
Fiscal Year
2008
Total Cost
$482,344
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Wang, Sheng-Bing; Venkatraman, Vidya; Crowgey, Erin L et al. (2018) Protein S-Nitrosylation Controls Glycogen Synthase Kinase 3? Function Independent of Its Phosphorylation State. Circ Res 122:1517-1531
Barth, Andreas S; Kumordzie, Ami; Tomaselli, Gordon F (2016) Orchestrated regulation of energy supply and energy expenditure: Transcriptional coexpression of metabolism, ion homeostasis, and sarcomeric genes in mammalian myocardium. Heart Rhythm 13:1131-1139
Barth, Andreas S; Tomaselli, Gordon F (2016) Gene scanning and heart attack risk. Trends Cardiovasc Med 26:260-5
O'Rourke, Brian; Liu, Ting; Foster, D Brian (2016) Seeing the Forest for the Trees. Circ Res 119:1170-1172
DeMazumder, Deeptankar; Kass, David A; O'Rourke, Brian et al. (2015) Cardiac resynchronization therapy restores sympathovagal balance in the failing heart by differential remodeling of cholinergic signaling. Circ Res 116:1691-9
Chung, Heaseung Sophia; Murray, Christopher I; Venkatraman, Vidya et al. (2015) Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection. Circ Res 117:846-57
Kirk, Jonathan A; Kass, David A (2015) Cellular and Molecular Aspects of Dyssynchrony and Resynchronization. Card Electrophysiol Clin 7:585-97
Kaushik, Gaurav; Spenlehauer, Alice; Sessions, Ayla O et al. (2015) Vinculin network-mediated cytoskeletal remodeling regulates contractile function in the aging heart. Sci Transl Med 7:292ra99
Kwon, Chulan; Tomaselli, Gordon F (2015) Coins of the realm in atrioventricular junction development. Circ Res 116:386-8
Tomaselli, Gordon F (2015) Introduction to a compendium on sudden cardiac death: epidemiology, mechanisms, and management. Circ Res 116:1883-6

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