Abstract

Core C is the Resources Core. All three Projectswill rely extensively on histological analysis of mouse hearts. Centralization of equipment, technical expertise and reagents will facilitate these activities and minimize the associated costs. Specific activities of Core C include (1) assistance with routine paraffin embedding and sectioning, (2) assistance with routine cryopreservation and sectioning, (3) assistance with standard histochemical staining (i.e., Hematoxylin and Eosin, Alcian blue, Masson's trichrome, Sirius red and fast green, von Kossa, etc), (4) assistance with in situ hybridizations, and (5) assistance with routine immune histology analyses (i.e., TUNEL, BrdU, sarcomeric actin, myosin heavy chain, etc). Given the heavy utilization of histologic analyses by all three projects, Core C is essential for the successful completion of the proposed experiments.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL085098-05
Application #
8280249
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2013-04-30
Budget Start
2011-05-01
Budget End
2013-04-30
Support Year
5
Fiscal Year
2011
Total Cost
$360,555
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202

  Publications

Lee, Seung-Hwan; Huang, Hu; Choi, Kangduk et al. (2014) ROCK1 isoform-specific deletion reveals a role for diet-induced insulin resistance. Am J Physiol Endocrinol Metab 306:E332-43
Dong, Yuanshu; Zhang, Lujuan; Bai, Yunpeng et al. (2014) Phosphatase of regenerating liver 2 (PRL2) deficiency impairs Kit signaling and spermatogenesis. J Biol Chem 289:3799-810
Shi, Jianjian; Surma, Michelle; Zhang, Lumin et al. (2013) Dissecting the roles of ROCK isoforms in stress-induced cell detachment. Cell Cycle 12:1492-500
Shi, Jianjian; Wei, Lei (2013) Rho kinases in cardiovascular physiology and pathophysiology: the effect of fasudil. J Cardiovasc Pharmacol 62:341-54
Shi, Jianjian; Wu, Xiangbing; Surma, Michelle et al. (2013) Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment. Cell Death Dis 4:e483
Chen, Hanying; Zhang, Wenjun; Sun, Xiaoxin et al. (2013) Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity. Development 140:1946-57
Wagner, Gregory R; Payne, R Mark (2013) Widespread and enzyme-independent N?-acetylation and N?-succinylation of proteins in the chemical conditions of the mitochondrial matrix. J Biol Chem 288:29036-45
Lafontant, Pascal J; Burns, Alan R; Grivas, Jamie A et al. (2012) The giant danio (D. aequipinnatus) as a model of cardiac remodeling and regeneration. Anat Rec (Hoboken) 295:234-48
Vincentz, Joshua W; VanDusen, Nathan J; Fleming, Andrew B et al. (2012) A Phox2- and Hand2-dependent Hand1 cis-regulatory element reveals a unique gene dosage requirement for Hand2 during sympathetic neurogenesis. J Neurosci 32:2110-20
Wagner, Gregory R; Pride, P Melanie; Babbey, Clifford M et al. (2012) Friedreich's ataxia reveals a mechanism for coordinate regulation of oxidative metabolism via feedback inhibition of the SIRT3 deacetylase. Hum Mol Genet 21:2688-97

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