Abstract

The purpose of this Core is to provide a central location for the supply of mice and services related to mouse care, maintenance, genotyping, and experimentation. Specifically this Core will offer the following services: 1. Managing the mouse colony that includes the oversight of the generation, maintenance, and supply of single and compound genetically altered mice 2. Harvesting DNA from mouse tail clips and performing PCR-based genotyping 3. Performing bone marrow transplantation 4. Preparing primary cultures of peritoneal macrophages and hepatocytes 5. Collecting plasma for isolation of lipoprotein fractions 6. Maintaining mice on specialized diets and administer pharmacological reagents as needed 7. Performing adenovirus infusions 8. Introducing radiolabeled macrophages and lipoprotein ligands into mice, and collecting plasma and tissue samples for kinetic studies Centralization of these facilities will permit an efficient use of resources for the Program, and will provide optimal quality control and standardization across Projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL086670-05
Application #
8238303
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
5
Fiscal Year
2011
Total Cost
$231,311
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Kim, Myung-Hee; de Beer, Maria C; Wroblewski, Joanne M et al. (2016) Impact of individual acute phase serum amyloid A isoforms on HDL metabolism in mice. J Lipid Res 57:969-79
Webb, Nancy R; De Beer, Maria C; Wroblewski, Joanne M et al. (2015) Deficiency of Endogenous Acute-Phase Serum Amyloid A Protects apoE-/- Mice From Angiotensin II-Induced Abdominal Aortic Aneurysm Formation. Arterioscler Thromb Vasc Biol 35:1156-65
Ji, Ailing; Wroblewski, Joanne M; Webb, Nancy R et al. (2014) Impact of phospholipid transfer protein on nascent high-density lipoprotein formation and remodeling. Arterioscler Thromb Vasc Biol 34:1910-6
De Beer, Maria C; Wroblewski, Joanne M; Noffsinger, Victoria P et al. (2014) Deficiency of endogenous acute phase serum amyloid A does not affect atherosclerotic lesions in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol 34:255-61
Meyer, Jason M; Ji, Ailing; Cai, Lei et al. (2014) Minimally oxidized LDL inhibits macrophage selective cholesteryl ester uptake and native LDL-induced foam cell formation. J Lipid Res 55:1648-56
Tang, Tao; Thompson, Joel C; Wilson, Patricia G et al. (2013) Decreased body fat, elevated plasma transforming growth factor-? levels, and impaired BMP4-like signaling in biglycan-deficient mice. Connect Tissue Res 54:5-13
Meyer, Jason M; Graf, Gregory A; van der Westhuyzen, Deneys R (2013) New developments in selective cholesteryl ester uptake. Curr Opin Lipidol 24:386-92
de Beer, Maria C; Wroblewski, Joanne M; Noffsinger, Victoria P et al. (2013) The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A. J Lipids 2013:283486
Kim, Myung-Hee; de Beer, Maria C; Wroblewski, Joanne M et al. (2013) SAA does not induce cytokine production in physiological conditions. Cytokine 61:506-12
Cai, Lei; Wang, Zhen; Meyer, Jason M et al. (2012) Macrophage SR-BI regulates LPS-induced pro-inflammatory signaling in mice and isolated macrophages. J Lipid Res 53:1472-81

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