Abstract

The In Vitro Models and Cell Culture Core continues to function as a resource for all projects in the PPG. It has provided relevant cell cultures to each of the projects and has provided cells, tissues, resources and expertise to collaborators around the world. Listed below are some ofthe contributions ofthe Core to the PPG. 1. Generation of in vitro models of pig airway epithelia. a. in vitro models of pig airway epithelia. b. in vitro models of pig alveolar epithelia. b. in vitro models of human and mouse airway epithelia for comparative studies. 2. Characterization of in vitro models and native epithelia. a. Electrophysiologic analysis. b. Morphologic evaluation. c. Expression profiles, mRNA and microRNA 3. Development of pig airway cell lines that grow as differentiated airway epithelia. 4. Research and development of new methods and models for the study of pig airway epithelia and submucosal glands. a. Optimize conditions for pig airway epithelia cultures. b. Develop methods for cultures of pig airway epithelia with goblet cell metaplasia. 5. Developing airway epithelia with siRNA knock down of genes. 6. Handling, characterizing and distribution of pig methacholine stimulated ASL. 7. Teaching investigators at the University of lowa and other institutions the methods for developing in vitro model systems. 8. Record keeping relevant to tissue acquisition, cell culture and phenotype. 9. Obtaining approval and record keeping for cell and animal studies from regulatory committees. 10. Provision of cells, plasmids, and viruses to investigators at other institutions. This Core brings expertise of a highly motivated and technically sophisticated staff to the benefit of PPG investigators. As a result, it allows investigators of the individual projects to focus their unique expertise on questions of high relevance to CF

Public Health Relevance

The In Vitro Models and Cell Culture Core functions as a resource for all projects in the PPG. It has provided relevant cell cultures to each of the projects and has provided cells^ tissues, resources and expertise to collaborators around the world.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL091842-06
Application #
8600369
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-09-19
Budget End
2014-06-30
Support Year
6
Fiscal Year
2013
Total Cost
$71,874
Indirect Cost
$24,275

  Institution

Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Meyerholz, David K; Sieren, Jessica C; Beck, Amanda P et al. (2018) Approaches to Evaluate Lung Inflammation in Translational Research. Vet Pathol 55:42-52
Meyerholz, David K; Stoltz, David A; Gansemer, Nick D et al. (2018) Lack of cystic fibrosis transmembrane conductance regulator disrupts fetal airway development in pigs. Lab Invest 98:825-838
Gray, Robert D; Hardisty, Gareth; Regan, Kate H et al. (2018) Delayed neutrophil apoptosis enhances NET formation in cystic fibrosis. Thorax 73:134-144
Meyerholz, David K; Beck, Amanda P; Goeken, J Adam et al. (2018) Glycogen depletion can increase the specificity of mucin detection in airway tissues. BMC Res Notes 11:763
Reznikov, Leah R; Meyerholz, David K; Kuan, Shin-Ping et al. (2018) Solitary Cholinergic Stimulation Induces Airway Hyperreactivity and Transcription of Distinct Pro-inflammatory Pathways. Lung 196:219-229
Meyerholz, David K; Reznikov, Leah R (2017) Simple and reproducible approaches for the collection of select porcine ganglia. J Neurosci Methods 289:93-98
Hisert, Katherine B; Heltshe, Sonya L; Pope, Christopher et al. (2017) Restoring Cystic Fibrosis Transmembrane Conductance Regulator Function Reduces Airway Bacteria and Inflammation in People with Cystic Fibrosis and Chronic Lung Infections. Am J Respir Crit Care Med 195:1617-1628
Paemka, Lily; McCullagh, Brian N; Abou Alaiwa, Mahmoud H et al. (2017) Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth. J Cyst Fibros 16:471-474
Meyerholz, David K; Ofori-Amanfo, Georgina K; Leidinger, Mariah R et al. (2017) Immunohistochemical Markers for Prospective Studies in Neurofibromatosis-1 Porcine Models. J Histochem Cytochem 65:607-618
Li, Xiaopeng; Vargas Buonfiglio, Luis G; Adam, Ryan J et al. (2017) Cystic Fibrosis Transmembrane Conductance Regulator Potentiation as a Therapeutic Strategy for Pulmonary Edema: A Proof-of-Concept Study in Pigs. Crit Care Med 45:e1240-e1246

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