Abstract

The long-term objectives of Project 3 are to determine, by in vivo and in vitro studies, the mechanisms by which HIV-1 mediates the functional and structural CNS pathology associated with pediatric AIDS. In addition, it is anticipated that these studies will provide new information on the contribution of microglia to the developing CNS. In vivo studies will employ immunocytochemical and histochemical methods specific for microglia and astrocytes to determine if HIV-1 interferes with normal gliogenesis. This will be done by comparing glial development in brains of fetuses from normal and from HIV-seropositive women. For the in vitro studies, selected and mixed populations of glial cells will be established to define the physiological parameters of cytokine production in culture Cytokine production will be determined by molecular biological and immunochemical methods. The contribution of specific populations of glial cells to cytokine production will be determined by in situ hybridization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Program Projects (P01)
Project #
1P01MH047667-01
Application #
3881633
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Grever, W E; Weidenheim, K M; Tricoche, M et al. (1997) Oligodendrocyte gene expression in the human fetal spinal cord during the second trimester of gestation. J Neurosci Res 47:332-40
Gilles, J M; Divon, M Y; Bentolila, E et al. (1997) Immunophenotypic characterization of human fetal liver hematopoietic stem cells during the midtrimester of gestation. Am J Obstet Gynecol 177:619-25
Chan, S O; Lyman, W D; Chiu, F C (1997) Temporal and spatial expression of glutamic acid decarboxylases in human fetal brain. Brain Res Mol Brain Res 46:318-20
Makman, M H; Lyman, W D; Dvorkin, B (1997) Presence and characterization of nociceptin (orphanin FQ) receptor binding in adult rat and human fetal hypothalamus. Brain Res 762:247-50
Cajigas, A; Suhrland, M; Harris, C et al. (1997) Correlation of the ratio of CD4+/CD8+ cells in lymph node fine needle aspiration biopsies with HIV clinical status. A preliminary study. Acta Cytol 41:1762-8
Grever, W E; Chiu, F C; Tricoche, M et al. (1996) Quantification of myelin basic protein in the human fetal spinal cord during the midtrimester of gestation. J Comp Neurol 376:306-14
Weidenheim, K M; Bodhireddy, S R; Rashbaum, W K et al. (1996) Temporal and spatial expression of major myelin proteins in the human fetal spinal cord during the second trimester. J Neuropathol Exp Neurol 55:734-45
Dickson, D W; Lee, S C (1996) Microglia in HIV-related CNS neuropathology: an update. J NeuroAIDS 1:57-83
Lee, S C; Dickson, D W; Casadevall, A (1996) Pathology of cryptococcal meningoencephalitis: analysis of 27 patients with pathogenetic implications. Hum Pathol 27:839-47
Aquino, D A; Padin, C; Perez, J M et al. (1996) Analysis of glial fibrillary acidic protein, neurofilament protein, actin and heat shock proteins in human fetal brain during the second trimester. Brain Res Dev Brain Res 91:1-10

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