CORE-B has resources to automatically scan, recognize and measure objects at high resolution directly from microscopic slides. It is a unique configuration of a Zeiss axioplan microscope with Ludl motorized stage, fine focus and filter changer; CCD camera; Perceptics Image Capture and Processing System; Local VAX cluster; and mass storage devices. The resource is connected to others via ethernet. Locally developed software systematically scans individual microscopic sections with automatic focusing; process images for features such as stained cell bodies, silver grains, and neuronal processes; generates montages from scanned areas of different objects; statistical analyses and representations of the data; data to LONI for alignment from serial sections. CORE-B will be linked to CORE-C to receive and process images from the confocal microscope and to CORE-E to send and receive data from the Laboratory of NeuroImaging. Projects 2, 3 and 6 will use the resource to enumerate particles like silver grains and HRP granules. Projects 4, 5 and 7 will use the resource to enumerate cells, fibers and silver grains. Project 1 will use the resource to collect images of axons for depiction.

Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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