Abstract

The Neuropathology/Cell Culture Core (Core B) has a central role in our Program Project. The approach of Core B will be multidisciplinary, using neuropathological and cell culture methods to evaluate mechanisms of ischemic brain injury. There are two main aims. In the first aim, the staff of Core B will be responsible for preparing all histologic sections for studies of focal and global ischemia, including sections processed for infarct volume determination, immunohistochemistry, DNA break staining (TUNEL), and in situ hybridization. The staff will assist in training personnel from Projects 1-5 in the associated data analysis. In the second aim, the staffof Core B will prepare and maintain all primary cell cultures for projects 1, 4, and 5.). Core B staff will also perform different models of induced-toxicity and perform immunocytochemistry, in vitro receptor autoradiography, cell binding assays, and pharmacological treatments on cultured cells. The staff will assist in training personnel from the various Projects in the data analysis associated with the cell cultures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
2P01NS020020-20
Application #
6825333
Study Section
Special Emphasis Panel (ZNS1-SRB-K (01))
Project Start
2003-09-30
Project End
2008-06-30
Budget Start
2003-09-30
Budget End
2004-06-30
Support Year
20
Fiscal Year
2003
Total Cost
$247,285
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Type
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Fonken, Laura K; Gaudet, Andrew D; Gaier, Kristopher R et al. (2016) MicroRNA-155 deletion reduces anxiety- and depressive-like behaviors in mice. Psychoneuroendocrinology 63:362-9
Ni, Xinli; Yang, Zeng-Jin; Wang, Bing et al. (2012) Early antioxidant treatment and delayed hypothermia after hypoxia-ischemia have no additive neuroprotection in newborn pigs. Anesth Analg 115:627-37
Ni, Xinli; Yang, Zeng-Jin; Carter, Erin L et al. (2011) Striatal neuroprotection from neonatal hypoxia-ischemia in piglets by antioxidant treatment with EUK-134 or edaravone. Dev Neurosci 33:299-311
Woodworth, K Nina; Palmateer, Julie; Swide, Joseph et al. (2011) Short- and long-term behavioral effects of exposure to 21%, 40% and 100% oxygen after perinatal hypoxia-ischemia in the rat. Int J Dev Neurosci 29:629-38
Yang, Sufang; Abrahams, Matthew S; Hurn, Patricia D et al. (2011) Local anesthetic Schwann cell toxicity is time and concentration dependent. Reg Anesth Pain Med 36:444-51
Nakano, Takaaki; Hurn, Patricia D; Herson, Paco S et al. (2010) Testosterone exacerbates neuronal damage following cardiac arrest and cardiopulmonary resuscitation in mouse. Brain Res 1357:124-30
Yang, Zeng-Jin; Carter, Erin L; Torbey, Michel T et al. (2010) Sigma receptor ligand 4-phenyl-1-(4-phenylbutyl)-piperidine modulates neuronal nitric oxide synthase/postsynaptic density-95 coupling mechanisms and protects against neonatal ischemic degeneration of striatal neurons. Exp Neurol 221:166-74
Ohata, Hiroto; Gebremedhin, Debebe; Narayanan, Jayashree et al. (2010) Onset of pulmonary ventilation in fetal sheep produces pial arteriolar constriction dependent on cytochrome p450 omega-hydroxylase activity. J Appl Physiol 109:412-7
Zhu, W; Wang, L; Zhang, L et al. (2010) Isoflurane preconditioning neuroprotection in experimental focal stroke is androgen-dependent in male mice. Neuroscience 169:758-69
Neigh, Gretchen N; Karelina, Kate; Glasper, Erica R et al. (2009) Anxiety after cardiac arrest/cardiopulmonary resuscitation: exacerbated by stress and prevented by minocycline. Stroke 40:3601-7

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