Abstract

The Neuropathology Core is designed to provide technical expertise and services relating to the histologic assessment and quantitation of demyelination within the CNS to complement methods of gross clinical assessment of disease for all members of the Program Project Grant (PPG). The overall goal of the PPG is to elucidate the cellular and molecular mechanisms of induction and progression of Theiler's virus induced demyelinating disease. A critical feature is the ability to assess and quantitate gross CNS pathology and demyelination under various disease conditions and in animals undergoing various regulatory treatments. The use of Dr. Mauro Dal Canto's longstanding expertise in neuropathology will allow uniform analysis and ready comparison among the different projects within the PPG and provide a focal point for collaboration among the members of the PPG. This Core will perform two vital functions for the execution of the PPG as a cohesive unit:
Aim 1 - Histopathologic analysis of CNS demyelination and inflammation.
Aim 2 - Preparation of organotypic CNS slice cultures for the assessment of the demyelinating capacity of various glial cell populations activated with various pro-inflammatory cytokines or directly by TMEV infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
2P01NS023349-17
Application #
6562289
Study Section
Special Emphasis Panel (ZNS1-SRB-A (03))
Project Start
2002-12-01
Project End
2007-11-30
Budget Start
2002-12-01
Budget End
2003-11-30
Support Year
17
Fiscal Year
2003
Total Cost
$144,969
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Ifergan, Igal; Davidson, Todd S; Kebir, Hania et al. (2017) Targeting the GM-CSF receptor for the treatment of CNS autoimmunity. J Autoimmun 84:1-11
Jeong, Su Ji; Cooper, John G; Ifergan, Igal et al. (2017) Intravenous immune-modifying nanoparticles as a therapy for spinal cord injury in mice. Neurobiol Dis 108:73-82
Richards, Maureen H; Getts, Meghann Teague; Podojil, Joseph R et al. (2011) Virus expanded regulatory T cells control disease severity in the Theiler's virus mouse model of MS. J Autoimmun 36:142-54
Jin, Young-Hee; Kang, Hyun Seok; Mohindru, Mani et al. (2011) Preferential induction of protective T cell responses to Theiler's virus in resistant (C57BL/6 x SJL)F1 mice. J Virol 85:3033-40
Getts, Meghann Teague; Richards, Maureen H; Miller, Stephen D (2010) A critical role for virus-specific CD8(+) CTLs in protection from Theiler's virus-induced demyelination in disease-susceptible SJL mice. Virology 402:102-11
Kang, Hyun Seok; Kim, Byung S (2010) Predominant clonal accumulation of CD8+ T cells with moderate avidity in the central nervous systems of Theiler's virus-infected C57BL/6 mice. J Virol 84:2774-86
Ercolini, A M; Miller, S D (2009) The role of infections in autoimmune disease. Clin Exp Immunol 155:1-15
Münz, Christian; Lünemann, Jan D; Getts, Meghann Teague et al. (2009) Antiviral immune responses: triggers of or triggered by autoimmunity? Nat Rev Immunol 9:246-58
Olson, Julie K; Miller, Stephen D (2009) The innate immune response affects the development of the autoimmune response in Theiler's virus-induced demyelinating disease. J Immunol 182:5712-22
Fan, Jilao; Son, Kyung-No; Arslan, Sevim Yildiz et al. (2009) Theiler's murine encephalomyelitis virus leader protein is the only nonstructural protein tested that induces apoptosis when transfected into mammalian cells. J Virol 83:6546-53

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