Abstract

The Immunohistochemical Imaging, Flow Cytometry, and Virus Preparation Core is designed to provide technical expertise and services relating to determining the phenotype and distribution of cells within the central nervous system as well as to provide virus stocks for members of the Program Project Grant (PPG). The overall goal of the PPG is to elucidate the cellular and molecular mechanisms of induction and progression of Theiler's virus-induced demyelinating disease. A critical feature is the ability to determine the phenotype of CNS-infiltrating cells as well as their spatial distribution during the development and progression of disease. Furthermore, investigations into disease regulation requires knowledge of how various treatments affect the normal cellular migration and accumulation patterns. This Core will perform three vital functions for the execution of the PPG as a cohesive unit:
Aim 1 - immunohistochemical analysis and imaging of CNS and peripheral lymphoid tissues.
Aim 2 - Oversight and management of the flow cytometry instrumentation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS023349-20
Application #
7557086
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2005-12-01
Budget End
2006-11-30
Support Year
20
Fiscal Year
2006
Total Cost
$144,717
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Ifergan, Igal; Davidson, Todd S; Kebir, Hania et al. (2017) Targeting the GM-CSF receptor for the treatment of CNS autoimmunity. J Autoimmun 84:1-11
Jeong, Su Ji; Cooper, John G; Ifergan, Igal et al. (2017) Intravenous immune-modifying nanoparticles as a therapy for spinal cord injury in mice. Neurobiol Dis 108:73-82
Richards, Maureen H; Getts, Meghann Teague; Podojil, Joseph R et al. (2011) Virus expanded regulatory T cells control disease severity in the Theiler's virus mouse model of MS. J Autoimmun 36:142-54
Jin, Young-Hee; Kang, Hyun Seok; Mohindru, Mani et al. (2011) Preferential induction of protective T cell responses to Theiler's virus in resistant (C57BL/6 x SJL)F1 mice. J Virol 85:3033-40
Kang, Hyun Seok; Kim, Byung S (2010) Predominant clonal accumulation of CD8+ T cells with moderate avidity in the central nervous systems of Theiler's virus-infected C57BL/6 mice. J Virol 84:2774-86
Getts, Meghann Teague; Richards, Maureen H; Miller, Stephen D (2010) A critical role for virus-specific CD8(+) CTLs in protection from Theiler's virus-induced demyelination in disease-susceptible SJL mice. Virology 402:102-11
Münz, Christian; Lünemann, Jan D; Getts, Meghann Teague et al. (2009) Antiviral immune responses: triggers of or triggered by autoimmunity? Nat Rev Immunol 9:246-58
Olson, Julie K; Miller, Stephen D (2009) The innate immune response affects the development of the autoimmune response in Theiler's virus-induced demyelinating disease. J Immunol 182:5712-22
Fan, Jilao; Son, Kyung-No; Arslan, Sevim Yildiz et al. (2009) Theiler's murine encephalomyelitis virus leader protein is the only nonstructural protein tested that induces apoptosis when transfected into mammalian cells. J Virol 83:6546-53
Jin, Young-Hee; Kang, Bongsu; Kim, Byung S (2009) Theiler's virus infection induces a predominant pathogenic CD4+ T cell response to RNA polymerase in susceptible SJL/J mice. J Virol 83:10981-92

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