Abstract

The Confocal and Electron Microscopy Facility is equipped for electron microscopy (EM) and light microscopy (LM) including confocal and live-cell imaging, including time-lapse video and calcium imaging systems. It has also been expanded during the previous funding period to include a Computer Imaging Facility for image processing and analysis. All 5 projects will use the LM-based systems and 3 will use EM. In addition to the equipment, the facility provides training and technical support. The facility is overseen by Dr. Leslie Tolbert and employs three staff members: 1) a Master's level Senior Research Specialist, Ms. Jansma, (60% effort on PPG and 40% for the Division) technically expert in EM and confocal microscopy, she maintains all of the scopes, trains new users and helps to develop protocols. 2) a part-time research technician, Ms. Potts (20% effort here and 80% effort on Project 2) provides training and assistance with the confocal and live-cell systems. 3) a part-time computer expert, Mr. Yuhas (75% effort on PPG and 25% effort in the Division) maintains computers, installs upgrades and trains users in software.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS028495-15
Application #
7090680
Study Section
Project Start
2005-07-01
Project End
2007-06-30
Budget Start
2005-07-01
Budget End
2007-06-30
Support Year
15
Fiscal Year
2005
Total Cost
$178,545
Indirect Cost

  Institution

Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721

  Publications

Srinivasan, Subhashini; Lance, Kimberley; Levine, Richard B (2012) Contribution of EAG to excitability and potassium currents in Drosophila larval motoneurons. J Neurophysiol 107:2660-71
Srinivasan, Subhashini; Lance, Kimberley; Levine, Richard B (2012) Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons. J Neurophysiol 107:1356-65
Mallory, Heather S; Gibson, Nicholas J; Hayashi, Jon H et al. (2011) Direct and glia-mediated effects of GABA on development of central olfactory neurons. Neuron Glia Biol 7:143-61
Schaefer, Jennifer E; Worrell, Jason W; Levine, Richard B (2010) Role of intrinsic properties in Drosophila motoneuron recruitment during fictive crawling. J Neurophysiol 104:1257-66
Gibson, Nicholas J; Tolbert, Leslie P; Oland, Lynne A (2009) Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system. PLoS One 4:e7222
Sztarker, Julieta; Strausfeld, Nicholas; Andrew, David et al. (2009) Neural organization of first optic neuropils in the littoral crab Hemigrapsus oregonensis and the semiterrestrial species Chasmagnathus granulatus. J Comp Neurol 513:129-50
Strausfeld, Nicholas J; Sinakevitch, Irina; Brown, Sheena M et al. (2009) Ground plan of the insect mushroom body: functional and evolutionary implications. J Comp Neurol 513:265-291
Oland, Lynne A; Biebelhausen, John P; Tolbert, Leslie P (2008) Glial investment of the adult and developing antennal lobe of Drosophila. J Comp Neurol 509:526-50
Hartwig, Cortnie L; Worrell, Jason; Levine, Richard B et al. (2008) Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor. Dev Neurobiol 68:1225-42
Abeytunga, D Thusitha U; Oland, Lynne; Somogyi, Arpad et al. (2008) Structural studies on the neutral glycosphingolipids of Manduca sexta. Bioorg Chem 36:70-6

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