Abstract

In order to facilitate Projects 2,3,4, and 5, and animal core laboratory has been established to do the following: provide a well-defined animal model of brain injury which produces nonfatal, focal and reproducible injury resulting in a histopathological picture similar to the clinical condition: handle and process all experimental and control animals for the experimenters; perform all surgeries on the animals under the most careful of conditions; perform tests of sensory-perceptual and motor functioning on the animals; perform behavior assessment of cognitive functions on the animals; and process tissue for histological tests. Non-penetrating, localized deformation of the cortex has been the mouse successful and extensively used rodent model. We will employ an electronically-controlled pneumatic impactor to achieve a closely controlled strike of the brain's cortical surface, in a fully anesthetized animal. This device produces precisely controlled brain contusions which are reproducible and which resemble many of the aspects of closed head injury seen clinically.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS031220-04
Application #
6243735
Study Section
Project Start
1997-04-01
Project End
1998-03-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Gebregziabher, Mulugeta; Hoel, David (2009) Applications of the Poly-K Statistical Test to Life-Time Cancer Bioassay Studies. Hum Ecol Risk Assess 15:858-875
Hatton, Jimmi; Kryscio, Richard; Ryan, Melody et al. (2006) Systemic metabolic effects of combined insulin-like growth factor-I and growth hormone therapy in patients who have sustained acute traumatic brain injury. J Neurosurg 105:843-52
Scheff, S W; Price, D A; Hicks, R R et al. (2005) Synaptogenesis in the hippocampal CA1 field following traumatic brain injury. J Neurotrauma 22:719-32
Sullivan, Patrick G; Rabchevsky, Alexander G; Keller, Jeffery N et al. (2004) Intrinsic differences in brain and spinal cord mitochondria: Implication for therapeutic interventions. J Comp Neurol 474:524-34
Dempsey, Robert J; Raghavendra Rao, Vemuganti L (2003) Cytidinediphosphocholine treatment to decrease traumatic brain injury-induced hippocampal neuronal death, cortical contusion volume, and neurological dysfunction in rats. J Neurosurg 98:867-73
Raghavendra Rao, Vemuganti L; Bowen, Kellie K; Dhodda, Vinay K et al. (2002) Gene expression analysis of spontaneously hypertensive rat cerebral cortex following transient focal cerebral ischemia. J Neurochem 83:1072-86
Guo, Jun-Tao; Yu, Jin; Grass, David et al. (2002) Inflammation-dependent cerebral deposition of serum amyloid a protein in a mouse model of amyloidosis. J Neurosci 22:5900-9
Adibhatla, Rao Muralikrishna; Hatcher, James F; Sailor, Kurt et al. (2002) Polyamines and central nervous system injury: spermine and spermidine decrease following transient focal cerebral ischemia in spontaneously hypertensive rats. Brain Res 938:81-6
Rao, V L; Dogan, A; Todd, K G et al. (2001) Antisense knockdown of the glial glutamate transporter GLT-1, but not the neuronal glutamate transporter EAAC1, exacerbates transient focal cerebral ischemia-induced neuronal damage in rat brain. J Neurosci 21:1876-83
Mottino, A D; Hoffman, T; Jennes, L et al. (2001) Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats. Am J Physiol Gastrointest Liver Physiol 280:G1261-73

Showing the most recent 10 out of 68 publications