A normal functioning nervous system depends upon the ability of neurons to recognize and synapse with their appropriate target cells during development. Although the cellular events involved in this type of cell interaction have been well described in both vertebrates and invertebrates, the underlying molecular mechanisms are poorly understood. Our long-term goal in this project is to understand the molecular basis of these neuronal recognition events. The approach we have taken is to use the genetics of Drosophila to identify genes and gene products that are involved in neuronal recognition. To this end, we have isolated mutations that alter the synaptic connections between identified neurons within a simple neuronal circuit. One of these mutations, called bendless (ben), appears to alter the recognition event leading to proper connectivity between two well characterized neurons of the circuit, the giant fiber and one of its post-synaptic targets, the TTM motoneuron. We have mapped the genomic location of the ben gene on a molecular walk through the region, and propose both to identify the ben transcription unit and to use molecular genetic and transformation techniques to study the role of the ben gene product in the target recognition process. We will determine which cells require ben function, and also misexpress the gene during development. We expect that these experiments will provide us with a better understanding of the target recognition process and its molecular basis, not only in Drosophila, but in higher vertebrates as well.

Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Zhang, Jingming; Lanuza, Guillermo M; Britz, Olivier et al. (2014) V1 and v2b interneurons secure the alternating flexor-extensor motor activity mice require for limbed locomotion. Neuron 82:138-50
Borowska, Joanna; Jones, Christopher T; Zhang, Han et al. (2013) Functional subpopulations of V3 interneurons in the mature mouse spinal cord. J Neurosci 33:18553-65
Levine, Ariel J; Lewallen, Kathryn A; Pfaff, Samuel L (2012) Spatial organization of cortical and spinal neurons controlling motor behavior. Curr Opin Neurobiol 22:812-21
Bonanomi, Dario; Chivatakarn, Onanong; Bai, Ge et al. (2012) Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals. Cell 148:568-82
Wang, Biao; Moya, Noel; Niessen, Sherry et al. (2011) A hormone-dependent module regulating energy balance. Cell 145:596-606
Bevins, Nicholas; Lemke, Greg; Reber, Michael (2011) Genetic dissection of EphA receptor signaling dynamics during retinotopic mapping. J Neurosci 31:10302-10
Alaynick, William A; Jessell, Thomas M; Pfaff, Samuel L (2011) SnapShot: spinal cord development. Cell 146:178-178.e1
Bai, Ge; Chivatakarn, Onanong; Bonanomi, Dario et al. (2011) Presenilin-dependent receptor processing is required for axon guidance. Cell 144:106-18
Grossmann, Katja S; Giraudin, Aurore; Britz, Olivier et al. (2010) Genetic dissection of rhythmic motor networks in mice. Prog Brain Res 187:19-37
Garcia-Campmany, Lidia; Stam, Floor J; Goulding, Martyn (2010) From circuits to behaviour: motor networks in vertebrates. Curr Opin Neurobiol 20:116-25

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