The Neuropathology Core, Core 2, will maintain a bank of central nervous system (CNS) tissues from children who died with HIV-1 infection, including both frozen and fixed tissues. Snap frozen tissue blocks are currently available from 25 cases, and there are also formalin-fixed, paraffin-embedded tissue blocks from these cases as well as from another 50 cases. It is anticipated that up to five new autopsy cases of children with AIDS, coming from the Pediatric AIDS Clinical Trials at University Hospital, will be accessioned by the tissue bank for each year of the project. Tissues will also be obtained from HIV-1-negative control cases. Furthermore, CNS tissue specimens will also be obtained from HIV-1- infected and HIV-1 negative adults who come to autopsy at University Hospital. Finally, tissues will be made available to the Core from the Neuropathology Division of Columbia-Presbyterian Medical Center in New York, both from HIV-1 infected and HIV-1 negative autopsies. The anticipated number of HIV-1 infected adult cases is up to 10 per year. The Core will supply tissue specimens to the various Projects as needed. In addition, the Core will provide neuropathology support and tissue immunocytochemistry and HIV-1 in situ-PCR services to the Projects. The PI, Dr. Leroy Sharer, will continue a survey of CNS tissues for the presence of HIV-1, using in situ-PCR. In addition, he will continue to monitor neuropathological changes in the pediatric cases coming to postmortem examination, with particular emphasis on the occurrence of HIV- 1 encephalitis and its relationship to anti-retroviral therapy.
| Schutt, Charles R; Gendelman, Howard E; Mosley, R Lee (2018) Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson's disease. Mol Neurodegener 13:26 |
| Sillman, Brady; Bade, Aditya N; Dash, Prasanta K et al. (2018) Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443 |
| Thomas, Midhun B; Gnanadhas, Divya Prakash; Dash, Prasanta K et al. (2018) Modulating cellular autophagy for controlled antiretroviral drug release. Nanomedicine (Lond) 13:2139-2154 |
| McMillan, JoEllyn; Szlachetka, Adam; Slack, Lara et al. (2018) Pharmacokinetics of a Long-Acting Nanoformulated Dolutegravir Prodrug in Rhesus Macaques. Antimicrob Agents Chemother 62: |
| Sillman, Brady; Woldstad, Christopher; Mcmillan, Joellyn et al. (2018) Neuropathogenesis of human immunodeficiency virus infection. Handb Clin Neurol 152:21-40 |
| Zhou, Tian; Su, Hang; Dash, Prasanta et al. (2018) Creation of a nanoformulated cabotegravir prodrug with improved antiretroviral profiles. Biomaterials 151:53-65 |
| Kevadiya, Bhavesh D; Ottemann, Brendan M; Thomas, Midhun Ben et al. (2018) Neurotheranostics as personalized medicines. Adv Drug Deliv Rev : |
| McMillan, JoEllyn; Szlachetka, Adam; Zhou, Tian et al. (2018) Pharmacokinetic testing of a first generation cabotegravir prodrug in rhesus macaques. AIDS : |
| Araínga, Mariluz; Edagwa, Benson; Mosley, R Lee et al. (2017) A mature macrophage is a principal HIV-1 cellular reservoir in humanized mice after treatment with long acting antiretroviral therapy. Retrovirology 14:17 |
| Gnanadhas, Divya Prakash; Dash, Prasanta K; Sillman, Brady et al. (2017) Autophagy facilitates macrophage depots of sustained-release nanoformulated antiretroviral drugs. J Clin Invest 127:857-873 |
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