Core B: Neuropathology and Tissue Culture Core JC virus (JCV) is well known as the cause of the human demyelinating disease progressive multifocal leukoencephalopathy (PML). In animals and in humans it has been associated with the production of brain tumors, most likely through mechanisms involving T-antigen a product of the JCV early genes. In human medulloblastomas, a frequent malignant brain tumor of children, our laboratory has also demonstrated JCV DNA and T antigen protein using PCR and immunocytochemistry. We have also developed a murine model of medulloblastoma in mice transgenic for the JCV archetypal early genes (JCV-CY mice). Because of the parallels between the human tumors and murine model, this program project proposes to focus on medulloblastomas and the relationship to JCV pathways. Within the overall project, Core B will perform pathologic evaluation of animals, tumors, and primary cultures from tumors generated by Core Component A: Experimental Animal Core. Core will also derive cell lines from primary tumor tissue and supply the individual projects with the necessary tissue and cell lines. This Core will contribute to the program project by providing a systematic, consistent and reliable mechanism for the tissue based analysis of the experimental animals, tumors and cell cultures utilized in the experiments. Integrating the pathologic and cell culture expertise with Projects 1,2 and 3 plus Core A will ensure that handling, storage and analysis of these specimens and cells is uniform and efficient.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
2P01NS036466-07A2
Application #
6825079
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2003-09-30
Project End
2008-05-31
Budget Start
2003-09-30
Budget End
2004-05-31
Support Year
7
Fiscal Year
2003
Total Cost
$259,805
Indirect Cost
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
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Urbanska, Katarzyna; Pannizzo, Paola; Grabacka, Maja et al. (2008) Activation of PPARalpha inhibits IGF-I-mediated growth and survival responses in medulloblastoma cell lines. Int J Cancer 123:1015-24

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