Core B: Neuropathology and Tissue Culture Core JC virus (JCV) is well known as the cause of the human demyelinating disease progressive multifocal leukoencephalopathy (PML). In animals and in humans it has been associated with the production of brain tumors, most likely through mechanisms involving T-antigen a product of the JCV early genes. In human medulloblastomas, a frequent malignant brain tumor of children, our laboratory has also demonstrated JCV DNA and T antigen protein using PCR and immunocytochemistry. We have also developed a murine model of medulloblastoma in mice transgenic for the JCV archetypal early genes (JCV-CY mice). Because of the parallels between the human tumors and murine model, this program project proposes to focus on medulloblastomas and the relationship to JCV pathways. Within the overall project, Core B will perform pathologic evaluation of animals, tumors, and primary cultures from tumors generated by Core Component A: Experimental Animal Core. Core will also derive cell lines from primary tumor tissue and supply the individual projects with the necessary tissue and cell lines. This Core will contribute to the program project by providing a systematic, consistent and reliable mechanism for the tissue based analysis of the experimental animals, tumors and cell cultures utilized in the experiments. Integrating the pathologic and cell culture expertise with Projects 1,2 and 3 plus Core A will ensure that handling, storage and analysis of these specimens and cells is uniform and efficient.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS036466-08
Application #
7553663
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2004-06-01
Budget End
2005-05-31
Support Year
8
Fiscal Year
2004
Total Cost
$256,055
Indirect Cost
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Johnson, Edward M; Daniel, Dianne C; Gordon, Jennifer (2013) The pur protein family: genetic and structural features in development and disease. J Cell Physiol 228:930-7
Riolfi, Mirko; Ferla, Rita; Del Valle, Luis et al. (2010) Leptin and its receptor are overexpressed in brain tumors and correlate with the degree of malignancy. Brain Pathol 20:481-9
Urbanska, Katarzyna; Pannizzo, Paola; Lassak, Adam et al. (2009) Estrogen receptor beta-mediated nuclear interaction between IRS-1 and Rad51 inhibits homologous recombination directed DNA repair in medulloblastoma. J Cell Physiol 219:392-401
Gualco, Elisa; Wang, Jin Ying; Del Valle, Luis et al. (2009) IGF-IR in neuroprotection and brain tumors. Front Biosci (Landmark Ed) 14:352-75
Rossi, Alessandra; Russo, Giuseppe; Puca, Andrew et al. (2009) The antiretroviral nucleoside analogue Abacavir reduces cell growth and promotes differentiation of human medulloblastoma cells. Int J Cancer 125:235-43
Brown, Meghan C; Staniszewska, Izabela; Lazarovici, Philip et al. (2008) Regulatory effect of nerve growth factor in alpha9beta1 integrin-dependent progression of glioblastoma. Neuro Oncol 10:968-80
Del Valle, Luis; White, Martyn K; Khalili, Kamel (2008) Potential mechanisms of the human polyomavirus JC in neural oncogenesis. J Neuropathol Exp Neurol 67:729-40
Perez-Liz, Georgina; Del Valle, Luis; Gentilella, Antonio et al. (2008) Detection of JC virus DNA fragments but not proteins in normal brain tissue. Ann Neurol 64:379-87
Fiorilli, Paul; Partridge, Darren; Staniszewska, Izabela et al. (2008) Integrins mediate adhesion of medulloblastoma cells to tenascin and activate pathways associated with survival and proliferation. Lab Invest 88:1143-56
Urbanska, Katarzyna; Pannizzo, Paola; Grabacka, Maja et al. (2008) Activation of PPARalpha inhibits IGF-I-mediated growth and survival responses in medulloblastoma cell lines. Int J Cancer 123:1015-24

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