This core facility provides analytical chemistry and histopathology support for Projects 1,2, and 3. The neurochemical analysis provided include vasoactive compounds and metabolites measured in serum, cerebrospinal fluid, and microdialysate samples. Amino acids including the nitric oxide related amino acids arginine and citrulline, the excitatory amino acids glutamate and aspartate, the volume regulatory amino acid taurine and the inhibitory amino acid glycine are measured by high performance liquid chromatography. The vasoactive purine adenosine which is elevated during bioenergetic distress is also measured by HPLC. Potassium in microdialysate is elevated with loss of membrane potential maintenance is measured by ion chromatography. The major breakdown products of nitric oxide, nitrate and nitrite, are measured using chemiluminescence. Vasoactive peptides such as endothelin are measured using enzyme- linked immunoassay. Histology support consists of photographic and digital documentation, gross examination, tissue processing, tissue sectioning, and staining. Contusion volume and neuronal cell counting is performed using image analysis software. Using intralumenal markers of capillary perfusion and immunohistochemical markers of capillary endothelial cells, the fraction of perfused capillaries will be measured morphologenically Immunohistochemical staining an also be performed as need; the current repertoire of immunohistochemical staining includes beta-amyloid, glucose and monocarboxylate transporters, endothelin, nitric oxide synthetase (NOS), coagulation factor 8, and 3-nitrotyrosine.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS038660-04
Application #
6613369
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2002-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
4
Fiscal Year
2002
Total Cost
$266,433
Indirect Cost
Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
Rubin, Maria Laura; Chan, Wenyaw; Yamal, Jose-Miguel et al. (2017) A joint logistic regression and covariate-adjusted continuous-time Markov chain model. Stat Med 36:4570-4582
Aisiku, Imoigele P; Yamal, Jose-Miguel; Doshi, Pratik et al. (2016) The incidence of ARDS and associated mortality in severe TBI using the Berlin definition. J Trauma Acute Care Surg 80:308-12
Aisiku, Imo P; Yamal, Jose-Miguel; Doshi, Pratik et al. (2016) Plasma cytokines IL-6, IL-8, and IL-10 are associated with the development of acute respiratory distress syndrome in patients with severe traumatic brain injury. Crit Care 20:288
Vedantam, Aditya; Yamal, Jose-Miguel; Rubin, Maria Laura et al. (2016) Progressive hemorrhagic injury after severe traumatic brain injury: effect of hemoglobin transfusion thresholds. J Neurosurg 125:1229-1234
Lazaridis, Christos; Yang, Ming; DeSantis, Stacia M et al. (2015) Predictors of intensive care unit length of stay and intracranial pressure in severe traumatic brain injury. J Crit Care 30:1258-62
Yamal, José-Miguel; Benoit, Julia S; Doshi, Pratik et al. (2015) Association of transfusion red blood cell storage age and blood oxygenation, long-term neurologic outcome, and mortality in traumatic brain injury. J Trauma Acute Care Surg 79:843-9
Yamal, Jose-Miguel; Rubin, M Laura; Benoit, Julia S et al. (2015) Effect of Hemoglobin Transfusion Threshold on Cerebral Hemodynamics and Oxygenation. J Neurotrauma 32:1239-45
Yamal, Jose-Miguel; Robertson, Claudia S; Rubin, M Laura et al. (2014) Enrollment of racially/ethnically diverse participants in traumatic brain injury trials: effect of availability of exception from informed consent. Clin Trials 11:187-94
Robertson, Claudia S; Yamal, Jose-Miguel; Tilley, Barbara C (2014) Erythropoietin for traumatic brain injury--reply. JAMA 312:1929
Robertson, Claudia S; Hannay, H Julia; Yamal, José-Miguel et al. (2014) Effect of erythropoietin and transfusion threshold on neurological recovery after traumatic brain injury: a randomized clinical trial. JAMA 312:36-47

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