The Core provides resources that are either directly shared by two or more projects or that promote project interaction. The PI is responsible for the Core and for assessing how each project interacts with the others. Glutamate clearance and energy metabolism derangement must be put into the context of patient selection, conduct of operative intervention, uniform description of tissue to be studied, and outcome regarding seizure control. The core will, therefore, be divided into four sections: Section 1: Preoperative definition of the patient population and presumed epileptogenic substrate. In this section, the PI will first define the patients that will undergo study, will obtain informed consent, will place electrodes in those patients requiring invasive study, and will make sure that all preoperative and qualitative and quantitative imaging and electrophysiology has been carried out (patient selection at conference, MRI, volumetrics, AVEEG, PET, MRS). Microdialysis assessment of glutamate will be provided both in vivo, when patients undergo invasive study, and in the slice. This data will be used correlatively in all the projects. Section: Intraoperative 13 study and tissue distribution. This involves digitized imaging of the brain intraoperatively to document the site of electrophysiological abnormalities and hippocampus in photographed, sectioned and numbered for investigator review. Section 3: Postoperative tissue characterization. This section is devoted to the neuronal and glial cell counts performed by Dr. Jung Kim which provides the cellular substrate for the metabolic and energy correlation studies. Section 4: Project coordination. The PI will provide coordination and data sharing among the projects assisted by an administrative associate who will maintain weekly conferences, coordinate patient movement among all the preoperative tests including the trips to Brookhaven National Laboratories. The administrator is also responsible for collecting and collating all data, coordinating interdisciplinary studies, and scheduling internal and external advisory meetings.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS039092-03
Application #
6495441
Study Section
Special Emphasis Panel (ZNS1)
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
3
Fiscal Year
2001
Total Cost
$225,543
Indirect Cost
Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520
Cavus, Idil; Widi, Gabriel A; Duckrow, Robert B et al. (2016) 50 Hz hippocampal stimulation in refractory epilepsy: Higher level of basal glutamate predicts greater release of glutamate. Epilepsia 57:288-97
Pan, J W; Duckrow, R B; Spencer, D D et al. (2013) Selective homonuclear polarization transfer for spectroscopic imaging of GABA at 7T. Magn Reson Med 69:310-6
Dericioglu, Nese; Garganta, Cheryl L; Petroff, Ognen A et al. (2008) Blockade of GABA synthesis only affects neural excitability under activated conditions in rat hippocampal slices. Neurochem Int 53:22-32
Cavus, Idil; Pan, Jullie W; Hetherington, Hoby P et al. (2008) Decreased hippocampal volume on MRI is associated with increased extracellular glutamate in epilepsy patients. Epilepsia 49:1358-66
Eid, Tore; Hammer, Janniche; Runden-Pran, Elise et al. (2007) Increased expression of phosphate-activated glutaminase in hippocampal neurons in human mesial temporal lobe epilepsy. Acta Neuropathol 113:137-52
Bjornsen, L P; Eid, T; Holmseth, S et al. (2007) Changes in glial glutamate transporters in human epileptogenic hippocampus: inadequate explanation for high extracellular glutamate during seizures. Neurobiol Dis 25:319-30
Malthankar-Phatak, Gauri H; de Lanerolle, Nihal; Eid, Tore et al. (2006) Differential glutamate dehydrogenase (GDH) activity profile in patients with temporal lobe epilepsy. Epilepsia 47:1292-9
Cavus, Idil; Kasoff, Willard S; Cassaday, Michael P et al. (2005) Extracellular metabolites in the cortex and hippocampus of epileptic patients. Ann Neurol 57:226-35
Pan, J W; Kim, J H; Cohen-Gadol, A et al. (2005) Regional energetic dysfunction in hippocampal epilepsy. Acta Neurol Scand 111:218-24
Pan, Jullie W; Takahashi, Kan (2005) Interdependence of N-acetyl aspartate and high-energy phosphates in healthy human brain. Ann Neurol 57:92-7

Showing the most recent 10 out of 28 publications