Abstract

The Histology Core is responsible for overseeing all histological procedures used in this Program Project Grant, including trouble-shooting immunohistochemical and in situ hybridization procedures in conjunction with personnel from the individual projects. A full-time Research Technician and a supervising Research Analyst (whose salary is provided by the Department of Cell and Molecular Physiology) will conduct many of the procedures listed in this proposal; they will be assisted by research staff from several projects within this application with other protocols. Alan R. Light will be as an advisor to the core. The Histology Facility has been responsible for a number of techniques that have allowed more efficient use of histological methods on Principal Investigators' past projects in a Program Project Grant, previously led by Edward R. Perl. The Histology core occupies 600 ft/2 in an area on the ground floor of the Department of Cell & Molecular Physiology, and it is easily accessible for Projects 1 and 3. This facility houses all the major equipment necessary for the histological procedures described in the projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS039420-02
Application #
6470115
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$185,991
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Garraway, Sandra M; Mendell, Lorne M (2007) Spinal cord transection enhances afferent-evoked inhibition in lamina II neurons and abolishes BDNF-induced facilitation of their sensory input. J Neurotrauma 24:379-90
Zhu, Weiguo; Oxford, Gerry S (2007) Phosphoinositide-3-kinase and mitogen activated protein kinase signaling pathways mediate acute NGF sensitization of TRPV1. Mol Cell Neurosci 34:689-700
Lin, Ting; Li, Kai; Zhang, Fei-Yu et al. (2007) Dissociation of spinal microglia morphological activation and peripheral inflammation in inflammatory pain models. J Neuroimmunol 192:40-8
Zhu, Weiguo; Xu, Pin; Cuascut, Fernando X et al. (2007) Activin acutely sensitizes dorsal root ganglion neurons and induces hyperalgesia via PKC-mediated potentiation of transient receptor potential vanilloid I. J Neurosci 27:13770-80
Light, Alan R; Wu, Ying; Hughen, Ronald W et al. (2006) Purinergic receptors activating rapid intracellular Ca increases in microglia. Neuron Glia Biol 2:125-138
Garraway, Sandra M; Anderson, Aileen J; Mendell, Lorne M (2005) BDNF-induced facilitation of afferent-evoked responses in lamina II neurons is reduced after neonatal spinal cord contusion injury. J Neurophysiol 94:1798-804
Arvanian, Victor L; Motin, Vladimir; Mendell, Lorne M (2005) Comparison of metabotropic glutamate receptor responses at segmental and descending inputs to motoneurons in neonatal rat spinal cord. J Pharmacol Exp Ther 312:669-77
Wu, Ying; Willcockson, Helen H; Maixner, William et al. (2004) Suramin inhibits spinal cord microglia activation and long-term hyperalgesia induced by formalin injection. J Pain 5:48-55
Liu, L; Zhu, W; Zhang, Z-S et al. (2004) Nicotine inhibits voltage-dependent sodium channels and sensitizes vanilloid receptors. J Neurophysiol 91:1482-91
Horton, April C; Ehlers, Michael D (2004) Secretory trafficking in neuronal dendrites. Nat Cell Biol 6:585-91

Showing the most recent 10 out of 23 publications