The context in which a sensory experience occurs can strongly influence whether it is perceived aspleasurable or aversive. In Drosophila, the volatile lipid pheromone cis-vaccenyl acetate (cVA) is producedby males and transferred to females during mating. When presented on eggs laid at food sources, it acts asan attractant. When presented on a mature female, it causes both instantaneous and long-term suppressionof male courtship. In both of these situations the presence of another odor dictates the behavioral responseto cVA. Our overall goal is to understand how olfactory context can change the immediate effects of apheromone and lead to long-term changes in behavior. In this grant we will define the circuitry that isinvolved in sensing cVA and courtship-stimulating pheromones and identify the mechanisms that integratepheromone signals to create appropriate modulation of courtship initiation. We will use genetically encodedprobes of neuronal activity to assess the contribution of local antenna! lobe circuits to association of thesecues. These experiments will provide insights into how odor information is processed and into howolfacotrymemory is formed. Research in invertebrate model systems has arguably been the driver of the field ofmolecular memory in mammals. The high degree of structural and molecular conservation in the olfactorysystem and in mechanisms of learning makes work on olfactory plasticity in Drosophila germane to humanhealth for the many developmental and acquired conditions that affect learning and memory.
Specific aims are:1. Define the olfactory neuron inputs that are required for sensing cVA and courtship-stimulating femalepheromones.2. Investigate the mechanisms of association between stimulatory pheromones and cVA in the antennallobe.3. Investigate the mechanisms of context integration in the antennal lobe.4. Characterize the role of dTrpAl, a non-specific cation channel, in suppression of male-male courtship(collaboration with Garrity Lab).The olfactory system of Drosophila and the molecular basis of learning in this organism are highlyhomologus to that of humans. Understanding of the cellular mechnaism of olfactory learning in this organismwill provide valuable information that can be used in designing therapeutics for human disorders of cognitionsuch as Alzheimer's disease and mental retardation.
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| Guo, Fang; Cerullo, Isadora; Chen, Xiao et al. (2014) PDF neuron firing phase-shifts key circadian activity neurons in Drosophila. Elife 3: |
| Li, Yue; Guo, Fang; Shen, James et al. (2014) PDF and cAMP enhance PER stability in Drosophila clock neurons. Proc Natl Acad Sci U S A 111:E1284-90 |
| Li, Yue; Rosbash, Michael (2013) Accelerated degradation of perS protein provides insight into light-mediated phase shifting. J Biol Rhythms 28:171-82 |
| Ni, Lina; Bronk, Peter; Chang, Elaine C et al. (2013) A gustatory receptor paralogue controls rapid warmth avoidance in Drosophila. Nature 500:580-4 |
| Shang, Yuhua; Donelson, Nathan C; Vecsey, Christopher G et al. (2013) Short neuropeptide F is a sleep-promoting inhibitory modulator. Neuron 80:171-83 |
| Perrat, Paola N; DasGupta, Shamik; Wang, Jie et al. (2013) Transposition-driven genomic heterogeneity in the Drosophila brain. Science 340:91-5 |
| Rinberg, Anatoly; Taylor, Adam L; Marder, Eve (2013) The effects of temperature on the stability of a neuronal oscillator. PLoS Comput Biol 9:e1002857 |
| Luo, Weifei; Li, Yue; Tang, Chih-Hang Anthony et al. (2012) CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila. Genes Dev 26:2536-49 |
| Tang, Lamont S; Taylor, Adam L; Rinberg, Anatoly et al. (2012) Robustness of a rhythmic circuit to short- and long-term temperature changes. J Neurosci 32:10075-85 |
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