Abstract

The histology and imaging core will provide essential services to the three projects that make up this program project grant. Specifically, it will section spinal cord specimens, store sections, perform routine immunohistological stains, perform routine tracing, histological stains and establish new protocols where needed. The Core will provide for the skills and equipment necessary to perform imaging and complex stereological counts and confocal analysis of transplants in the spinal cord. This equipment is expensive and highly specialized, thus making such a shared arrangement more practical. Dr. Dan Peterson will serve as a consultant in the operation of this core, thereby adding considerable expertise pertaining to confocal and sterology applications;he will also help with the interpretation of spinal cord transplants. The experiments carried out within the program project could not be done efficiently without the services of the Histology and Immunology Core (Core B).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS057778-05
Application #
8235951
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
5
Fiscal Year
2011
Total Cost
$64,511
Indirect Cost

  Institution

Name
Cedars-Sinai Medical Center
Department
Type
DUNS #
075307785
City
Los Angeles
State
CA
Country
United States
Zip Code
90048

  Publications

Nichols, Nicole L; Satriotomo, Irawan; Allen, Latoya L et al. (2017) Mechanisms of Enhanced Phrenic Long-Term Facilitation in SOD1G93A Rats. J Neurosci 37:5834-5845
Nichols, Nicole L; Mitchell, Gordon S (2016) Quantitative assessment of integrated phrenic nerve activity. Respir Physiol Neurobiol 226:81-6
Jones, Jeffrey R; Zhang, Su-Chun (2016) Engineering human cells and tissues through pluripotent stem cells. Curr Opin Biotechnol 40:133-138
Chen, Hong; Qian, Kun; Chen, Wei et al. (2015) Human-derived neural progenitors functionally replace astrocytes in adult mice. J Clin Invest 125:1033-42
Nikodemova, Maria; Small, Alissa L; Smith, Stephanie M C et al. (2014) Spinal but not cortical microglia acquire an atypical phenotype with high VEGF, galectin-3 and osteopontin, and blunted inflammatory responses in ALS rats. Neurobiol Dis 69:43-53
Gowing, Geneviève; Shelley, Brandon; Staggenborg, Kevin et al. (2014) Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats. Neuroreport 25:367-72
Dale, E A; Ben Mabrouk, F; Mitchell, G S (2014) Unexpected benefits of intermittent hypoxia: enhanced respiratory and nonrespiratory motor function. Physiology (Bethesda) 29:39-48
Nichols, N L; Johnson, R A; Satriotomo, I et al. (2014) Neither serotonin nor adenosine-dependent mechanisms preserve ventilatory capacity in ALS rats. Respir Physiol Neurobiol 197:19-28
Nichols, Nicole L; Gowing, Genevieve; Satriotomo, Irawan et al. (2013) Intermittent hypoxia and stem cell implants preserve breathing capacity in a rodent model of amyotrophic lateral sclerosis. Am J Respir Crit Care Med 187:535-42
Dale, Erica A; Mitchell, Gordon S (2013) Spinal vascular endothelial growth factor (VEGF) and erythropoietin (EPO) induced phrenic motor facilitation after repetitive acute intermittent hypoxia. Respir Physiol Neurobiol 185:481-8

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