Abstract

This Core provides an essential function for the overall scientific execution of the performance of the individual projects. A main purpose of the Animal Core is to maintain and insure the genetic integrity of the large number of unique genetically altered mouse lines available for the use of each member of the Program Project. Mice from each line will be maintained and genotyped by the Core and provided to the individual investigators for expansion as dictated by the experimental design. The duties of this core include responsibility for the breeding program, weaning pups and genotyping. Responsibility for adjusting the census of each mouse line in anticipation ofthe needs ofthe Pi's such that expansion ofthe individual lines can be accomplished to facilitate timely experiments. The core is also responsible for the generation of new transgenic lines required for the successful demonstration of the mechanisms underiying the hypotheses tested in each project. Lastly, the core is responsible for insuring the sterile maintenance of the bone marrow chimeric mice and the extent of chimerism prior to use by the individual projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS064932-03
Application #
8325557
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-09-01
Budget End
2012-08-31
Support Year
3
Fiscal Year
2011
Total Cost
$241,445
Indirect Cost

  Institution

Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195

  Publications

Hwang, Mihyun; Bergmann, Cornelia C (2018) Alpha/Beta Interferon (IFN-?/?) Signaling in Astrocytes Mediates Protection against Viral Encephalomyelitis and Regulates IFN-?-Dependent Responses. J Virol 92:
Phares, Timothy W; DiSano, Krista D; Stohlman, Stephen A et al. (2016) CXCL13 promotes isotype-switched B cell accumulation to the central nervous system during viral encephalomyelitis. Brain Behav Immun 54:128-139
Butchi, Niranjan; Kapil, Parul; Puntambekar, Shweta et al. (2015) Myd88 Initiates Early Innate Immune Responses and Promotes CD4 T Cells during Coronavirus Encephalomyelitis. J Virol 89:9299-312
Hwang, Mihyun; Phares, Timothy W; Hinton, David R et al. (2015) Distinct CD4 T-cell effects on primary versus recall CD8 T-cell responses during viral encephalomyelitis. Immunology 144:374-386
Kapil, Parul; Stohlman, Stephen A; Hinton, David R et al. (2014) PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis. J Neuroimmunol 270:1-12
Butchi, Niranjan B; Hinton, David R; Stohlman, Stephen A et al. (2014) Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages. J Virol 88:1051-64
de Aquino, Maria Teresa P; Kapil, Parul; Hinton, David R et al. (2014) IL-27 limits central nervous system viral clearance by promoting IL-10 and enhances demyelination. J Immunol 193:285-94
Savarin, Carine; Bergmann, Cornelia C; Hinton, David R et al. (2013) MMP-independent role of TIMP-1 at the blood brain barrier during viral encephalomyelitis. ASN Neuro 5:e00127
Phares, Timothy W; DiSano, Krista D; Hinton, David R et al. (2013) IL-21 optimizes T cell and humoral responses in the central nervous system during viral encephalitis. J Neuroimmunol 263:43-54
de Aquino, Maria Teresa P; Puntambekar, Shweta S; Savarin, Carine et al. (2013) Role of CD25(+) CD4(+) T cells in acute and persistent coronavirus infection of the central nervous system. Virology 447:112-20

Showing the most recent 10 out of 18 publications