Prompted by the finding in rodents that local sleep, as assessed through OFF period detection in multiunit recordings, is accompanied in the local EEG by slow activity, we will establish if local sleep also occurs in humans, and if it is related to intense learning involving circumscribed regions of cortex. Preliminary results presented in this Project indicate that in humans, as in rodents, cortical areas heavily involved in learning a task show a progressive increase in the occurrence of slow activity (2-9Hz) detected with hdEEG. To better characterize these effects, we will use an implicit rotation learning task that relies on a sensorimotor-parietal network, and a declarative visual sequence learning task with a prominent working memory component that relies on frontal and occipito-temporal cortex.
In Aim 1, we will establish if intense training in each task results in the occurrence of local, regionally specific, slow activity in the spontaneous EEG.
In Aim 2, we will test if intense training leads to specific impairment in performance tests involving the same brain regions, and if errors are associated, on a trial-by-trial basis, with local EEG slow activity in specific areas and at specific times, depending on the nature of the task and the stage of the test.
In Aim 3, we will test whether local EEG slow activity in wake is due to intense synaptic plasticity (""""""""tiredness""""""""), like sleep, or instead is merely """"""""fatigue"""""""" due to intense activity. We will also establish if sleep is necessary, rather than just rest, is necessary and sufficient to counteract local EEG slowing and tiredness. If successful, these studies, complemented by those of Project I (flies) and II (mice), will open new ways to investigating the occurrence, consequences and opportunities for the prevention of local sleep in neurological and psychiatric disorders.
| Bellesi, Michele; de Vivo, Luisa; Koebe, Samuel et al. (2018) Sleep and Wake Affect Glycogen Content and Turnover at Perisynaptic Astrocytic Processes. Front Cell Neurosci 12:308 |
| Bourdon, Allen K; Spano, Giovanna Maria; Marshall, William et al. (2018) Metabolomic analysis of mouse prefrontal cortex reveals upregulated analytes during wakefulness compared to sleep. Sci Rep 8:11225 |
| Bellesi, Michele; Haswell, John Douglas; de Vivo, Luisa et al. (2018) Myelin modifications after chronic sleep loss in adolescent mice. Sleep 41: |
| Honjoh, Sakiko; Sasai, Shuntaro; Schiereck, Shannon S et al. (2018) Regulation of cortical activity and arousal by the matrix cells of the ventromedial thalamic nucleus. Nat Commun 9:2100 |
| Darracq, Matthieu; Funk, Chadd M; Polyakov, Daniel et al. (2018) Evoked Alpha Power is Reduced in Disconnected Consciousness During Sleep and Anesthesia. Sci Rep 8:16664 |
| Marinelli, Lucio; Quartarone, Angelo; Hallett, Mark et al. (2017) The many facets of motor learning and their relevance for Parkinson's disease. Clin Neurophysiol 128:1127-1141 |
| Siclari, Francesca; Tononi, Giulio (2017) Local aspects of sleep and wakefulness. Curr Opin Neurobiol 44:222-227 |
| Boly, Melanie; Jones, Benjamin; Findlay, Graham et al. (2017) Altered sleep homeostasis correlates with cognitive impairment in patients with focal epilepsy. Brain 140:1026-1040 |
| de Vivo, Luisa; Bellesi, Michele; Marshall, William et al. (2017) Ultrastructural evidence for synaptic scaling across the wake/sleep cycle. Science 355:507-510 |
| Honjoh, Sakiko; de Vivo, Luisa; Okuno, Hiroyuki et al. (2017) Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse Cortex. Front Neural Circuits 11:60 |
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