A core facility which allows the expertise and the material from these cell culture and rodent models to be made available to the wider MSSM community will be an essential resource for groups studying the effects of alcohol on the liver. To complement these well-established and useful systems, new models, enabling novel approaches to studying the genetics and pathophysiology of ALD are sought. Zebrafish are renowned as a genetically tractable vertebrate system for studying embryonic development and for modeling disease. A major advantage to using zebrafish is that large numbers of embryos - hundreds to thousands on a given day - can be generated and treated with alcohol in a cost-effective and rapid manner. A state-of-the art zebrafish facility has been established at MSSM and is being used to develop genetic models of liver disease. Thus, the zebrafish component of the Models Core represents a new direction in ALD research, and including it as a core facility will enable researchers with little or no zebrafish experience to use this novel system. The combination of in vitro and in vivo resources provided by the two components of this core provide a unique environment in which to study several aspects of alcohol-generated oxidative stress liver injury.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Exploratory Grants (P20)
Project #
5P20AA017067-03
Application #
8120921
Study Section
Special Emphasis Panel (ZAA1)
Project Start
Project End
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
3
Fiscal Year
2010
Total Cost
$71,776
Indirect Cost
Name
Icahn School of Medicine at Mount Sinai
Department
Type
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Ge, Xiaodong; Arriazu, Elena; Magdaleno, Fernando et al. (2018) High Mobility Group Box-1 Drives Fibrosis Progression Signaling via the Receptor for Advanced Glycation End Products in Mice. Hepatology 68:2380-2404
Magdaleno, Fernando; Blajszczak, Chuck C; Nieto, Natalia (2017) Key Events Participating in the Pathogenesis of  Alcoholic Liver Disease. Biomolecules 7:
Arriazu, Elena; Ge, Xiaodong; Leung, Tung-Ming et al. (2017) Signalling via the osteopontin and high mobility group box-1 axis drives the fibrogenic response to liver injury. Gut 66:1123-1137
Laitman, Benjamin M; Asp, Linnéa; Mariani, John N et al. (2016) The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination. PLoS Biol 14:e1002467
Renault, Thibaud T; Luna-Vargas, Mark P A; Chipuk, Jerry E (2016) Mouse Liver Mitochondria Isolation, Size Fractionation, and Real-time MOMP Measurement. Bio Protoc 6:
Kocabayoglu, Peri; Zhang, David Y; Kojima, Kensuke et al. (2016) Induction and contribution of beta platelet-derived growth factor signalling by hepatic stellate cells to liver regeneration after partial hepatectomy in mice. Liver Int 36:874-82
Magdaleno, Fernando; Arriazu, Elena; Ruiz de Galarreta, Marina et al. (2016) Cartilage oligomeric matrix protein participates in the pathogenesis of liver fibrosis. J Hepatol 65:963-971
Kocabayoglu, Peri; Lade, Abigale; Lee, Youngmin A et al. (2015) ?-PDGF receptor expressed by hepatic stellate cells regulates fibrosis in murine liver injury, but not carcinogenesis. J Hepatol 63:141-7
Renault, Thibaud T; Floros, Konstantinos V; Elkholi, Rana et al. (2015) Mitochondrial shape governs BAX-induced membrane permeabilization and apoptosis. Mol Cell 57:69-82
Hasegawa, Daisuke; Calvo, Veronica; Avivar-Valderas, Alvaro et al. (2015) Epithelial Xbp1 is required for cellular proliferation and differentiation during mammary gland development. Mol Cell Biol 35:1543-56

Showing the most recent 10 out of 90 publications