The Biochemistry Core supports projects associated with the Center for Pediatric Research by providing consultation and technical support for protein-protein interaction screening, multi-analyte detection, and lipid profiling. The Core will continue to perform BioID as a cutting-edge approach to screen for protein-protein interactions. Furthermore, the Core will expand its technical abilities and provide services for multi-analyte detection and quantification of proteins as well as gas chromatography-mass spectrometry (GC/MS) for detection and quantification of fatty acids and sterols. The Core will also provide assistance in the preparation of manuscripts and funding applications as they relate to the services provided. By providing these services in- house, the core will directly interface with the investigators during experimental design, protocol establishment, troubleshooting and execution as well as organize any further analysis outside of the core. The purpose of the Biochemistry Core is to support the goals of its users, namely independent scientists studying how biochemical processes on stem/progenitor cell plasticity influence the basis of human health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103620-08
Application #
10004072
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2013-09-01
Project End
2023-08-31
Budget Start
2020-09-01
Budget End
2021-08-31
Support Year
8
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Sanford Research/Usd
Department
Type
DUNS #
050113252
City
Sioux Falls
State
SD
Country
United States
Zip Code
57104
Brudvig, J J; Cain, J T; Sears, R M et al. (2018) MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network. Sci Rep 8:13278
Roux, Kyle J; Kim, Dae In; Burke, Brian et al. (2018) BioID: A Screen for Protein-Protein Interactions. Curr Protoc Protein Sci 91:19.23.1-19.23.15
Hussain, Sajjad; Bedekovics, Tibor; Liu, Qiuying et al. (2018) UCH-L1 bypasses mTOR to promote protein biosynthesis and is required for MYC-driven lymphomagenesis in mice. Blood 132:2564-2574
Brudvig, J J; Cain, J T; Schmidt-Grimminger, G G et al. (2018) MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation. Mol Neurobiol 55:8388-8402
Anderson, Ruthellen H; Kerkvliet, Jason G; Otta, Jaelin J et al. (2018) Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem Cell Res 33:95-99
Lucido, Christopher T; Callejas-Valera, Juan L; Colbert, Paul L et al. (2018) ?2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC. Oncogenesis 7:81
McKenzie, Casey W; Preston, Claudia C; Finn, Rozzy et al. (2018) Strain-specific differences in brain gene expression in a hydrocephalic mouse model with motile cilia dysfunction. Sci Rep 8:13370
Miszuk, Jacob M; Xu, Tao; Yao, Qingqing et al. (2018) Functionalization of PCL-3D Electrospun Nanofibrous Scaffolds for Improved BMP2-Induced Bone Formation. Appl Mater Today 10:194-202
Anderson, Ruthellen H; Francis, Kevin R (2018) Modeling rare diseases with induced pluripotent stem cell technology. Mol Cell Probes 40:52-59
Amatya, Christina; Radichev, Ilian A; Ellefson, Jacob et al. (2018) Self-Transducible Bimodal PDX1-FOXP3 Protein Lifts Insulin Secretion and Curbs Autoimmunity, Boosting Tregs in Type 1 Diabetic Mice. Mol Ther 26:184-198

Showing the most recent 10 out of 59 publications