Abstract

High-throughput 'omics analysis platforms are the technologies of choice for analyzing complex mixtures derived from tissues and biological matrices such as liver, feces, intestine, blood and urine. To maximize our capability for deciphering the mechanism and therapy for liver injury, nutrition and gut:liver interactions, and liver:environment/toxin/drug interactions, we propose an OMICS Core in this Hepatobiology and Toxicology COBRE proposal. Our OMICS Core is composed of three major components including Metabolomics Core, Proteomics Core, and Bioinformatics. The overarching goal of the OMICS Core is to support the proposed COBRE research. This goal will be met through application of state of the art separations science and mass spectrometry techniques to analyze research samples containing complex mixtures of metabolites, proteins and protein fragments. This goal is achieved through the following aims:
Specific Aim 1 : Application of liquid chromatography mass spectrometry (LC-MS), comprehensive two- dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) and integrated laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) platforms for quantitative analysis of metabolites, metal ions, and metal-protein complex in biological samples.
Specific Aim 2 : Application of high-resolution multidimensional liquid chromatography mass spectrometry (MDLC-MS) and affinity enrichment methods for targeted protein quantification.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM113226-01
Application #
8813877
Study Section
Special Emphasis Panel (ZGM1-TWD-A (C1))
Project Start
2016-06-10
Project End
2021-03-31
Budget Start
2014-12-01
Budget End
2015-11-30
Support Year
1
Fiscal Year
2016
Total Cost
$346,275
Indirect Cost
$121,275

  Institution

Name
University of Louisville
Department
Type
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40208

  Publications

Hein, David W; Zhang, Xiaoyan; Doll, Mark A (2018) Role of N-acetyltransferase 2 acetylation polymorphism in 4, 4'-methylene bis (2-chloroaniline) biotransformation. Toxicol Lett 283:100-105
Hein, David W; Fakis, Giannoulis; Boukouvala, Sotiria (2018) Functional expression of human arylamine N-acetyltransferase NAT1*10 and NAT1*11 alleles: a mini review. Pharmacogenet Genomics 28:238-244
Liang, Yaqin; Lang, Anna L; Zhang, Jian et al. (2018) Exposure to Vinyl Chloride and Its Influence on Western Diet-Induced Cardiac Remodeling. Chem Res Toxicol 31:482-493
Kharbanda, Kusum K; Ronis, Martin J J; Shearn, Colin T et al. (2018) Role of Nutrition in Alcoholic Liver Disease: Summary of the Symposium at the ESBRA 2017 Congress. Biomolecules 8:
Ghosh Dastidar, Shubha; Warner, Jeffrey B; Warner, Dennis R et al. (2018) Rodent Models of Alcoholic Liver Disease: Role of Binge Ethanol Administration. Biomolecules 8:
Gosney, Julie A; Wilkey, Daniel W; Merchant, Michael L et al. (2018) Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner. J Biol Chem 293:5895-5908
Atay, Safinur; Wilkey, Daniel W; Milhem, Mohammed et al. (2018) Insights into the Proteome of Gastrointestinal Stromal Tumors-Derived Exosomes Reveals New Potential Diagnostic Biomarkers. Mol Cell Proteomics 17:495-515
Schuster, Susanne; Johnson, Casey D; Hennebelle, Marie et al. (2018) Oxidized linoleic acid metabolites induce liver mitochondrial dysfunction, apoptosis, and NLRP3 activation in mice. J Lipid Res 59:1597-1609
Lang, Anna L; Chen, Liya; Poff, Gavin D et al. (2018) Vinyl chloride dysregulates metabolic homeostasis and enhances diet-induced liver injury in mice. Hepatol Commun 2:270-284
Pandit, Harshul; Li, Yan; Li, Xuanyi et al. (2018) Enrichment of cancer stem cells via ?-catenin contributing to the tumorigenesis of hepatocellular carcinoma. BMC Cancer 18:783

Showing the most recent 10 out of 68 publications