The goals of the COBRE Center for Redox Biology and Cardiovascular Disease are to establish a nationally recognized advanced research and training program in redox biology regulation of cardiovascular disease and to facilitate the development of junior faculty research programs to achieve major independent research funding. The development of Core C, the Redox Molecular Signaling Core, will provide COBRE investigators and trainees with access to modern equipment for the detection and quantification of reactive oxygen species, reactive nitrogen species, and reactive sulfur species. Additionally, this facility will enable the analysis of redox- dependent control of protein function through redox proteomics and metabolomics, provide a centralized facility for cell culture hypoxia studies, and provide expertise for establishing cell culture models of redox signaling. This range of analytical techniques to assess redox signaling will be unmatched for a single facility in Louisiana. Establishment of the Redox Molecular Signaling Core will be accomplished through the formation of two distinct sub-cores, the Analytical Redox Biology Sub-Core and the Molecular Signaling Sub-Core, each building on existing services currently offered at LSU Health Shreveport. Establishment of the Analytical Redox Biology Sub-Core will occur through adding functionality to existing core equipment for high performance liquid chromatography (HPLC) and mass spectrometry (MS) currently associated with the Research Core Facility. The additional of key equipment will enable high quality, quantitative redox measurements at a centralized facility with dedicated technical staff. The Molecular Signaling Sub-Core will be formed through the addition of key equipment and expertise to core facilities available through the Department of Pathology. This core will provide a centralized facility for assistance with molecular biology and for establishing cell culture models of redox signaling and will provide access to vital equipment for modeling hypoxic cell injury in culture and for protein analysis using high throughput, automated capillary electrophoresis. The faculty and staff of the Redox Molecular Signaling Core will work closely with the COBRE investigators to optimize experimental design, perform analytical measurements, provide training on the use of core equipment, and assist with data collection and interpretation. These services will provide an invaluable benefit to the COBRE investigators to advance their individual projects while enhancing the infrastructure of local redox-biology research.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM121307-04
Application #
10086876
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2018-02-01
Project End
2023-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
4
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Louisiana State University Hsc Shreveport
Department
Type
DUNS #
095439774
City
Shreveport
State
LA
Country
United States
Zip Code
71103
Chen, Jie; Shen, Xinggui; Pardue, Sibile et al. (2018) The Ataxia telangiectasia-mutated and Rad3-related protein kinase regulates cellular hydrogen sulfide concentrations. DNA Repair (Amst) :
Shrestha, Bandana; Prasai, Priya K; Kaskas, Amir M et al. (2018) Differential arterial and venous endothelial redox responses to oxidative stress. Microcirculation 25:e12486
Prasai, Priya K; Shrestha, Bandana; Orr, A Wayne et al. (2018) Decreases in GSH:GSSG activate vascular endothelial growth factor receptor 2 (VEGFR2) in human aortic endothelial cells. Redox Biol 19:22-27
Al-Yafeai, Zaki; Yurdagul Jr, Arif; Peretik, Jonette M et al. (2018) Endothelial FN (Fibronectin) Deposition by ?5?1 Integrins Drives Atherogenic Inflammation. Arterioscler Thromb Vasc Biol 38:2601-2614