Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project was to optimize protocols for the analysis of gene expression in paraformaldehyde fixed monkey brain tissue.
The specific aims of the project were (1) To modify available protocols to extract total RNA from fixed brain tissue, (2) To optimize protocols for DNA microarray and real time RT-PCR to use RNA extracted from paraformaldehyde fixed brain tissues, and (3) To analyze differential gene expression in the rhesus monkey brain in fixed tissues obtained at six months and one year after traumatic brain injury.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015567-10
Application #
7959614
Study Section
Special Emphasis Panel (ZRR1-RI-8 (02))
Project Start
2009-06-01
Project End
2010-05-31
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
10
Fiscal Year
2009
Total Cost
$12,392
Indirect Cost

  Institution

Name
University of South Dakota
Department
Neurosciences
Type
Schools of Medicine
DUNS #
929930808
City
Vermillion
State
SD
Country
United States
Zip Code
57069

  Publications

Burrell, Brian D (2017) Comparative biology of pain: What invertebrates can tell us about how nociception works. J Neurophysiol 117:1461-1473
Robertson, James M; Achua, Justin K; Smith, Justin P et al. (2017) Anxious behavior induces elevated hippocampal Cb2 receptor gene expression. Neuroscience 352:273-284
Novick, Andrew M; Mears, Mackenzie; Forster, Gina L et al. (2016) Adolescent social defeat alters N-methyl-D-aspartic acid receptor expression and impairs fear learning in adulthood. Behav Brain Res 304:51-9
Smith, Justin P; Prince, Melissa A; Achua, Justin K et al. (2016) Intensity of anxiety is modified via complex integrative stress circuitries. Psychoneuroendocrinology 63:351-61
Robertson, James M; Prince, Melissa A; Achua, Justin K et al. (2015) Nuance and behavioral cogency: How the Visible Burrow System inspired the Stress-Alternatives Model and conceptualization of the continuum of anxiety. Physiol Behav 146:86-97
Ranek, Mark J; Zheng, Hanqiao; Huang, Wei et al. (2015) Genetically induced moderate inhibition of 20S proteasomes in cardiomyocytes facilitates heart failure in mice during systolic overload. J Mol Cell Cardiol 85:273-81
Hahn, Elizabeth; Burrell, Brian (2015) Pentylenetetrazol-induced seizure-like behavior and neural hyperactivity in the medicinal leech. Invert Neurosci 15:177
Novick, Andrew M; Forster, Gina L; Hassell, James E et al. (2015) Increased dopamine transporter function as a mechanism for dopamine hypoactivity in the adult infralimbic medial prefrontal cortex following adolescent social stress. Neuropharmacology 97:194-200
Ranek, Mark J; Kost Jr, Curtis K; Hu, Chengjun et al. (2014) Muscarinic 2 receptors modulate cardiac proteasome function in a protein kinase G-dependent manner. J Mol Cell Cardiol 69:43-51
Watt, Michael J; Roberts, Christina L; Scholl, Jamie L et al. (2014) Decreased prefrontal cortex dopamine activity following adolescent social defeat in male rats: role of dopamine D2 receptors. Psychopharmacology (Berl) 231:1627-36

Showing the most recent 10 out of 171 publications