Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Core B is the Molecular Biology Core. This core will serve all of the cobre projects by providing general cloning and expression of recombinant proteins, recombinant adenovirus production, Southern, Northern and Western analysis. In addition, Core B will design primers and probes for real-time PCR and DNA seqeuence analysis along with co-immunoprecipitation assay development. In addition, this core will perform all preparative work required by the COBRE projects for the subsequent analysis and identification of proteins and protein profiling. The University of Nevada has a well-established proteomics facility, the Nevada Proteomics Center, that can adequately perform all the necessary protein analysis such as protein mass and sequence determination.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015581-08
Application #
7609795
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
2007-06-01
Project End
2008-05-31
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
8
Fiscal Year
2007
Total Cost
$243,392
Indirect Cost

  Institution

Name
University of Nevada Reno
Department
Pharmacology
Type
Schools of Medicine
DUNS #
146515460
City
Reno
State
NV
Country
United States
Zip Code
89557

  Publications

Ba, Mariam A; Surina, Jeffrey; Singer, Cherie A et al. (2017) Knockdown of subunit 3 of the COP9 signalosome inhibits C2C12 myoblast differentiation via NF-KappaB signaling pathway. BMC Pharmacol Toxicol 18:47
Duan, Dayue Darrel (2013) Phenomics of cardiac chloride channels. Compr Physiol 3:667-92
Forrest, Abigail S; Joyce, Talia C; Huebner, Marissa L et al. (2012) Increased TMEM16A-encoded calcium-activated chloride channel activity is associated with pulmonary hypertension. Am J Physiol Cell Physiol 303:C1229-43
von Bartheld, Christopher S (2012) Distribution of Particles in the Z-axis of Tissue Sections: Relevance for Counting Methods. Neuroquantology 10:66-75
Angermann, Jeff E; Forrest, Abigail S; Greenwood, Iain A et al. (2012) Activation of Ca2+-activated Cl- channels by store-operated Ca2+ entry in arterial smooth muscle cells does not require reverse-mode Na+/Ca2+ exchange. Can J Physiol Pharmacol 90:903-21
Li, Chuanwei; Pei, Fang; Zhu, Xiaoshan et al. (2012) Circulating microRNAs as novel and sensitive biomarkers of acute myocardial Infarction. Clin Biochem 45:727-32
Wiggins, Larisa M; Kuta, A; Stevens, James C et al. (2012) A novel phenotype for the dynein heavy chain mutation Loa: altered dendritic morphology, organelle density, and reduced numbers of trigeminal motoneurons. J Comp Neurol 520:2757-73
Duan, Dayue Darrel (2011) The ClC-3 chloride channels in cardiovascular disease. Acta Pharmacol Sin 32:675-84
He, Xuyu; Gao, Xiuren; Peng, Longyun et al. (2011) Atrial fibrillation induces myocardial fibrosis through angiotensin II type 1 receptor-specific Arkadia-mediated downregulation of Smad7. Circ Res 108:164-75
Xiang, Sunny Yang; Ye, Linda L; Duan, Li-lu Marie et al. (2011) Characterization of a critical role for CFTR chloride channels in cardioprotection against ischemia/reperfusion injury. Acta Pharmacol Sin 32:824-33

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