Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The antiviral role of RNA interference (RNAi) has been well established in plant and lower animal systems. The goal of this research is to determine if RNAi is an important defense against viruses infecting mammals. Most plant-infecting viruses encode suppressors of RNAi to ensure host invasion. We are systematically screening several animal virus genomes in collaboration with other NCV researchers for the presence of genes with RNAi suppressor activity. The viruses to be tested include a Rhabdovirus (VSV) in collaboration with the Pattnaik lab, a Flavivirus (Dengue) in collaboration with the Zhang lab, a member of the Arteriviridae (PRRSV) in collaboration with the Osorio lab and Human papillomavirus (HPV) in collaboration with the Angeletti lab. Two different approaches are being used to test the central hypothesis that RNAi is an integral part of the complex antiviral defense system in mammals. First, a plant based transient assay has been used to screen proteins from various mammal-infecting viruses for potential suppressors. These have included a systematic analysis a Rhabdovirus (VSV) in collaboration with the Pattnaik lab; a Flavivirus (Dengue) in collaboration with the Zhang lab; and a member of the Arteriviridae (PRRSV) in collaboration with the Osorio lab. Several candidate viral genes were identified with possible suppressor activity. Second, animal cell lines stably expressing p19, a suppressor with cross-kingdom silencing suppression activity, were generated and screened for enhanced susceptibility to several different mammalian cell infecting viruses including HIV in collaboration with the Wood lab.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015635-08
Application #
7609843
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
2007-05-01
Project End
2008-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
8
Fiscal Year
2007
Total Cost
$46,447
Indirect Cost

  Institution

Name
University of Nebraska Lincoln
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
555456995
City
Lincoln
State
NE
Country
United States
Zip Code
68588

  Publications

De Castro, Cristina; Klose, Thomas; Speciale, Immacolata et al. (2018) Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate molecular modeling approaches. Proc Natl Acad Sci U S A 115:E44-E52
Yuan, Qi; Telling, Glenn; Bartelt-Hunt, Shannon L et al. (2018) Dehydration of Prions on Environmentally Relevant Surfaces Protects Them from Inactivation by Freezing and Thawing. J Virol 92:
Liu, Yilin; Jones, Clinton (2016) Regulation of Notch-mediated transcription by a bovine herpesvirus 1 encoded protein (ORF2) that is expressed in latently infected sensory neurons. J Neurovirol 22:518-28
Langenfeld, Katie A; Shikiya, Ronald A; Kincaid, Anthony E et al. (2016) Incongruity between Prion Conversion and Incubation Period following Coinfection. J Virol 90:5715-23
Liu, Yilin; Hancock, Morgan; Workman, Aspen et al. (2016) ?-Catenin, a Transcription Factor Activated by Canonical Wnt Signaling, Is Expressed in Sensory Neurons of Calves Latently Infected with Bovine Herpesvirus 1. J Virol 90:3148-59
De Castro, Cristina; Speciale, Immacolata; Duncan, Garry et al. (2016) N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. Angew Chem Int Ed Engl 55:654-8
Destache, Christopher J; Mandal, Subhra; Yuan, Zhe et al. (2016) Topical Tenofovir Disoproxil Fumarate Nanoparticles Prevent HIV-1 Vaginal Transmission in a Humanized Mouse Model. Antimicrob Agents Chemother 60:3633-9
Lingel, Amy; Ehlers, Erica; Wang, Qianli et al. (2016) Kaposi's Sarcoma-Associated Herpesvirus Reduces Cellular Myeloid Differentiation Primary-Response Gene 88 (MyD88) Expression via Modulation of Its RNA. J Virol 90:180-8
Liu, Fang; Huang, Yunlong; Zhang, Fang et al. (2015) Macrophages treated with particulate matter PM2.5 induce selective neurotoxicity through glutaminase-mediated glutamate generation. J Neurochem 134:315-26
Lai, Siqiang; Zhang, Min; Xu, Dongsheng et al. (2015) Direct reprogramming of induced neural progenitors: a new promising strategy for AD treatment. Transl Neurodegener 4:7

Showing the most recent 10 out of 429 publications