Abstract

This research core application describes a plan to establish a Flow Cytometry Core Facility that will support the current and future research of the multi-investigator project. The Flow Cytometry Core Facility will also serve the HSC research community, and provide a critical resource for establishing the HSC as a center of biomedical research excellence (COBRE). The Core Facility will use existing instrumentation including an analytic flow cytometer (FACScan) and a cell sorter (FACStar Plus). Funding is requested for a leser and computer upgrade for the FACStar Plus, partial salary support for a Core Director, full salary support for a Technical Director, and service contracts for both instruments. This proposal describes features of the Flow Cytometry Core Facility including the management plan, the fee structure, a description of services, the quality assurance plan, and strategies to maintain and expand the facility over the life of the project and beyond. In addition, the proposal includes a description of how the facility will enable the COBRE investigators and mentors to fulfill their research objectives.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR016440-01
Application #
6553284
Study Section
Special Emphasis Panel (ZRR1)
Project Start
2001-09-14
Project End
2006-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
West Virginia University
Department
Type
DUNS #
191510239
City
Morgantown
State
WV
Country
United States
Zip Code
26506

  Publications

Nichols, Cody E; Shepherd, Danielle L; Hathaway, Quincy A et al. (2018) Reactive oxygen species damage drives cardiac and mitochondrial dysfunction following acute nano-titanium dioxide inhalation exposure. Nanotoxicology 12:32-48
Shumar, Stephanie A; Kerr, Evan W; Geldenhuys, Werner J et al. (2018) Nudt19 is a renal CoA diphosphohydrolase with biochemical and regulatory properties that are distinct from the hepatic Nudt7 isoform. J Biol Chem 293:4134-4148
Bedenbaugh, M N; O'Connell, R C; Lopez, J A et al. (2018) Kisspeptin, gonadotrophin-releasing hormone and oestrogen receptor ? colocalise with neuronal nitric oxide synthase neurones in prepubertal female sheep. J Neuroendocrinol 30:
Rodgers, H M; Huffman, V J; Voronina, V A et al. (2018) The role of the Rx homeobox gene in retinal progenitor proliferation and cell fate specification. Mech Dev 151:18-29
Brooks, Celine; Snoberger, Aaron; Belcastro, Marycharmain et al. (2018) Archaeal Unfoldase Counteracts Protein Misfolding Retinopathy in Mice. J Neurosci 38:7248-7254
Grisez, Brian T; Ray, Justin J; Bostian, Phillip A et al. (2018) Highly metastatic K7M2 cell line: A novel murine model capable of in vivo imaging via luciferase vector transfection. J Orthop Res :
Voronkova, Maria A; Luanpitpong, Sudjit; Rojanasakul, Liying Wang et al. (2017) SOX9 Regulates Cancer Stem-Like Properties and Metastatic Potential of Single-Walled Carbon Nanotube-Exposed Cells. Sci Rep 7:11653
Chakraborty, Sreeparna; Castranova, Vincent; Perez, Miriam K et al. (2017) Nanoparticles-induced apoptosis of human airway epithelium is mediated by proNGF/p75NTRsignaling. J Toxicol Environ Health A 80:53-68
Takahashi, Hideyuki; Suzuki, Yutaka; Mohamed, Junaith S et al. (2017) Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats. Exp Gerontol 92:56-66
Deng, Wentao; McLaughlin, Sarah L; Klinke, David J (2017) Quantifying spontaneous metastasis in a syngeneic mouse melanoma model using real time PCR. Analyst 142:2945-2953

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