This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The immediate aim of this project is to determine the effect of pyrethroid insecticides on calcium homeostasis in mouse caudal epididymal sperm and the effect of these compounds on viability. Recent results indicate that voltage-sensitive calcium channels are target sites for pyrethroid insecticides. Thus, in the proposed studies at Salve Regina University, a primarily undergraduate university, the hypothesis that pyrethroid insecticides modify calcium homeostasis via modification of voltage-sensitive calcium channels will be investigated. Specifically, we will use mouse caudal epididymal sperm to examine the effects of pyrethroids on sperm motility, calcium influx, and the acrosome reaction;processes that are regulated by intracellular calcium influx via voltage-sensitive calcium channels. It is expected that pyrethroids will act as antagonists and will result in sterility due to inhibition of calcium influx via calcium channels associated with mammalian spermatozoa. A combination of behavioral and fluorescent biochemical assays in addition to more rigorous electrophysiological methods will be used to assess the effects of pyrethroids on voltage-sensitive calcium channels using mouse caudal epididymal sperm. These methods have previously been shown to be useful in the assessment of ion permeability changes induced by channel antagonists. Pyrethroids are the predominant class of insecticides used to control several medically important insects that vector human diseases. These potent insecticides have a wide spectrum of use and are effective in controlling a variety of insect pests in the prevention of insect-mediated human and animal disease transmission. The increased spread of the aforementioned diseases will likely result in increased efforts to control medically important insects and increased exposure situations for individuals in urban environments. Thus, it is critical that these insecticides be evaluated for the potential adverse effects on reproduction.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR016457-09
Application #
7960137
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2009-05-04
Project End
2010-04-30
Budget Start
2009-05-04
Budget End
2010-04-30
Support Year
9
Fiscal Year
2009
Total Cost
$87,575
Indirect Cost
Name
University of Rhode Island
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881
Taylor, David L; Calabrese, Nicholas M (2018) Mercury content of blue crabs (Callinectes sapidus) from southern New England coastal habitats: Contamination in an emergent fishery and risks to human consumers. Mar Pollut Bull 126:166-178
Chen, Xiaodi; Hovanesian, Virginia; Naqvi, Syed et al. (2018) Systemic infusions of anti-interleukin-1? neutralizing antibodies reduce short-term brain injury after cerebral ischemia in the ovine fetus. Brain Behav Immun 67:24-35
Paquin, Karissa L; Howlett, Niall G (2018) Understanding the Histone DNA Repair Code: H4K20me2 Makes Its Mark. Mol Cancer Res 16:1335-1345
Hahn, Mark E; Karchner, Sibel I; Merson, Rebeka R (2017) Diversity as Opportunity: Insights from 600 Million Years of AHR Evolution. Curr Opin Toxicol 2:58-71
Preiss, Matthew R; Cournoyer, Eily; Paquin, Karissa L et al. (2017) Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers. Bioconjug Chem 28:2729-2736
Tiwari, Rakesh K; Brown, Alex; Sadeghiani, Neda et al. (2017) Design, Synthesis, and Evaluation of Dasatinib-Amino Acid and Dasatinib-Fatty Acid Conjugates as Protein Tyrosine Kinase Inhibitors. ChemMedChem 12:86-99
Shimpi, Prajakta C; More, Vijay R; Paranjpe, Maneesha et al. (2017) Hepatic Lipid Accumulation and Nrf2 Expression following Perinatal and Peripubertal Exposure to Bisphenol A in a Mouse Model of Nonalcoholic Liver Disease. Environ Health Perspect 125:087005
Malloy, Thomas E; Kinney, Lorin (2017) Implications for the Self Determine Benevolence and Self-Protection in Intergroup Relations. Self Identity 16:171-193
Vierra, David A; Garzon, Jada L; Rego, Meghan A et al. (2017) Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure. Oncotarget 8:76443-76457
Wan, Jerry; Lin, Fuquan; Zhang, Wei et al. (2017) Novel approaches to vitiligo treatment via modulation of mTOR and NF-?B pathways in human skin melanocytes. Int J Biol Sci 13:391-400

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