This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Maintenance of genomic integrity is essential for all organisms, and cells respond to DNA damage with a tightly orchestrated sequence of events that coordinates cell cycle arrest and DNA repair. The overall objective of this project is to advance our understanding of this process, using S. cerevisiae as a model system to study the function of Bcp1, an essential protein in yeast. Bcp1 is the fungal homolog of BCCIP, a protein originally identified by its association with tumor suppressor BRCA2, and cell cycle regulator CDKN1A (p21). In human cells, BCCIP promotes cell cycle arrest following DNA damage, and participates in homologous recombination repair in conjunction with BRCA2. To begin to elucidate the role of Bcp1 in the S. cerevisiae DNA damage response, we will examine the hypothesis that Bcp1 localizes to sites of DNA damage and contributes to checkpoint activation leading to cell cycle arrest.
Two Specific Aims are proposed: 1) Analyze Bcp1 localization and association with chromatin-bound protein complexes in cells treated with DNA damaging agents, and define how loss of Bcp1 impacts DNA repair or cell survival and 2) Evaluate a role for Bcp1 in checkpoint activation in response to DNA damage. The results of this study will provide insight as to the biological function of Bcp1 and its contribution to the critically important DNA damage response.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016457-11
Application #
8360082
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$95,673
Indirect Cost
Name
University of Rhode Island
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881
Taylor, David L; Calabrese, Nicholas M (2018) Mercury content of blue crabs (Callinectes sapidus) from southern New England coastal habitats: Contamination in an emergent fishery and risks to human consumers. Mar Pollut Bull 126:166-178
Chen, Xiaodi; Hovanesian, Virginia; Naqvi, Syed et al. (2018) Systemic infusions of anti-interleukin-1? neutralizing antibodies reduce short-term brain injury after cerebral ischemia in the ovine fetus. Brain Behav Immun 67:24-35
Paquin, Karissa L; Howlett, Niall G (2018) Understanding the Histone DNA Repair Code: H4K20me2 Makes Its Mark. Mol Cancer Res 16:1335-1345
Hahn, Mark E; Karchner, Sibel I; Merson, Rebeka R (2017) Diversity as Opportunity: Insights from 600 Million Years of AHR Evolution. Curr Opin Toxicol 2:58-71
Preiss, Matthew R; Cournoyer, Eily; Paquin, Karissa L et al. (2017) Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers. Bioconjug Chem 28:2729-2736
Tiwari, Rakesh K; Brown, Alex; Sadeghiani, Neda et al. (2017) Design, Synthesis, and Evaluation of Dasatinib-Amino Acid and Dasatinib-Fatty Acid Conjugates as Protein Tyrosine Kinase Inhibitors. ChemMedChem 12:86-99
Shimpi, Prajakta C; More, Vijay R; Paranjpe, Maneesha et al. (2017) Hepatic Lipid Accumulation and Nrf2 Expression following Perinatal and Peripubertal Exposure to Bisphenol A in a Mouse Model of Nonalcoholic Liver Disease. Environ Health Perspect 125:087005
Malloy, Thomas E; Kinney, Lorin (2017) Implications for the Self Determine Benevolence and Self-Protection in Intergroup Relations. Self Identity 16:171-193
Vierra, David A; Garzon, Jada L; Rego, Meghan A et al. (2017) Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure. Oncotarget 8:76443-76457
Wan, Jerry; Lin, Fuquan; Zhang, Wei et al. (2017) Novel approaches to vitiligo treatment via modulation of mTOR and NF-?B pathways in human skin melanocytes. Int J Biol Sci 13:391-400

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