This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous research indicates that testosterone enhances the survival of newly proliferated neurons in the adult hippocampus, and increased survival of hippocampal neurons may correlate with improved spatial memory. Therefore, adult neurogenesis may be the mechanism by which testosterone improves spatial working memory. We examined both performance on a working-reference memory version of the 8-arm radial maze (RAM) and 30-day hippocampal cell survival using castrated adult male rats. Subjects were injected with BrdU (200 mg/kg) to assess the survival of newly proliferated cells within the hippocampus. Beginning the following day, rats were run once daily for 29 days on the RAM. The number of both working memory errors (WME) and reference memory errors (RME) was scored. Each day prior to maze testing, rats were injected with either 0.5 mg of testosterone propionate (n = 10) or 0.1ml of oil vehicle (n = 10). BrdU-labeled cells were stained and scored on brain sections. Testosterone-injected subjects performed significantly fewer WMEs than did controls during the first ten days of testing. In contrast, testosterone had no significant effect on RMEs. This indicates that testosterone improves working memory but not reference memory. Surprisingly, testosterone had no effect on cell survival. This may have been because learning on the maze elevated neurogenesis levels in the control subjects. These results suggest that enhanced hippocampal cell survival may not be the mechanism by which testosterone improves spatial memory.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR016462-09
Application #
8168166
Study Section
Special Emphasis Panel (ZRR1-RI-7 (01))
Project Start
2010-08-01
Project End
2011-05-31
Budget Start
2010-08-01
Budget End
2011-05-31
Support Year
9
Fiscal Year
2010
Total Cost
$99,485
Indirect Cost
Name
University of Vermont & St Agric College
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Wagner, Benjamin A; Braddick, Valerie C; Batson, Christopher G et al. (2018) Effects of testosterone dose on spatial memory among castrated adult male rats. Psychoneuroendocrinology 89:120-130
Mireault, Gina C; Crockenberg, Susan C; Heilman, Keri et al. (2018) Social, cognitive, and physiological aspects of humour perception from 4 to 8 months: Two longitudinal studies. Br J Dev Psychol 36:98-109
Mireault, Gina C; Rainville, Brady S; Laughlin, Breanna (2018) Push or Carry? Pragmatic Opportunities for Language Development in Strollers vs. Backpacks. Infancy 23:616-624
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Nock, Adam M; Wargo, Matthew J (2016) Choline Catabolism in Burkholderia thailandensis Is Regulated by Multiple Glutamine Amidotransferase 1-Containing AraC Family Transcriptional Regulators. J Bacteriol 198:2503-14
Spritzer, M D; Curtis, M G; DeLoach, J P et al. (2016) Sexual interactions with unfamiliar females reduce hippocampal neurogenesis among adult male rats. Neuroscience 318:143-56
Hinkle, Karen L; Anderson, Chad C; Forkey, Blake et al. (2016) Exposure to the lampricide 3-trifluoromethyl-4-nitrophenol results in increased expression of carbohydrate transporters in Saccharomyces cerevisiae. Environ Toxicol Chem 35:1727-32
Kirshenbaum, Ari; Green, John; Fay, Michael et al. (2015) Reinforcer devaluation as a consequence of acute nicotine exposure and withdrawal. Psychopharmacology (Berl) 232:1583-94
Bentley, P A; Wall, E H; Dahl, G E et al. (2015) Responses of the mammary transcriptome of dairy cows to altered photoperiod during late gestation. Physiol Genomics 47:488-99
Mireault, Gina C; Crockenberg, Susan C; Sparrow, John E et al. (2015) Laughing matters: Infant humor in the context of parental affect. J Exp Child Psychol 136:30-41

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