This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. LCC's outreach core research has three components, 1) learn research techniques in isolation and characterization of Protein Disulfide Isomerase (PDI) genes from Arabidopsis with Dr. David Christopher of the College of Tropical Agriculture and Human Resources, UH Manoa, as mentor; 2) mentor LCC students in research methodology and 3) conduct outreach activities to K-12 students in modern life sciences education. Three outreach activities have been performed in 2005 with INBRE support. First, two Campbell High School students Daisy M. Ramos and Erica Bailey K. Suzuki, worked for their high school science fair project in Fall 2004. The students used Real Time PCR to investigate potential contamination of non-transgenic papaya seed lots in UH seed lab with transgenic papaya seeds. Both Erica and Daisy worked in different labs and interacted with a number of scientists in the field, including Prof. Richard Manshardt. Their project entitled Determining Levels of Contamination in Non-Transgenic Carica papaya through the Use of Real Time PCR-Made Standard? received the following award in the Hawaii State Science and Engineering Fair held in April 4-6, 2005: American Chemical Society, Hawai`i Section Award, 2nd Place ($50) Hawai`i Agriculture research Center Award ($50 and certificate) Gamma Sigma Delta, the Honor Society of Agriculture Award ($100 and certificate) Hawaii Veterinary Medical Association Award ($100) The second activity involved demonstration of DNA from fruits and construction of a 3-D DNA model to 20 kingergarten students in Hokulani elementary School. The last outreach activity involved a field visit by eight students from the Honors Biology class and their teacher from Mililani High School (MHS) to LCC. Students participated in a two-day laboratory experience in which the students learned about how DNA is used to help solve crime in forensic laboratories. In addition to laboratory safety, students learned several molecular techniques, including plasmid isolation, restriction digestion, agarose gel electrophoresis and photodocumentation of results.
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