Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator.
Specific Aims : To clone Listeria monocytogenes inlA and inlB genes for overproduction/purification of internalin proteins. To subject cell cultures to purified internalin proteins and monitor internalization of each protein. To potentially adhere drugs to internalin proteins for cell internalization. Hypothesis: Internalin proteins derived from inlA and inlB genes of Listeria monocytogenes will be effective at triggering internalization in human cell lines and thus, may be useful as a means of drug delivery.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016469-10
Application #
8167497
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2010-05-01
Project End
2011-04-30
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
10
Fiscal Year
2010
Total Cost
$46,290
Indirect Cost

  Institution

Name
University of Nebraska Medical Center
Department
Genetics
Type
Schools of Medicine
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198

  Publications

Barta, Cody L; Liu, Huizhan; Chen, Lei et al. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Sci Data 5:180005
Liu, Huizhan; Chen, Lei; Giffen, Kimberlee P et al. (2018) Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells. Front Mol Neurosci 11:356
Wehrkamp, Cody J; Natarajan, Sathish Kumar; Mohr, Ashley M et al. (2018) miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family. RNA Biol 15:391-403
Lopez, Wilfredo; Page, Alexis M; Carlson, Darby J et al. (2018) Analysis of immune-related genes during Nora virus infection of Drosophila melanogaster using next generation sequencing. AIMS Microbiol 4:123-139
Azadmanesh, Jahaun; Trickel, Scott R; Borgstahl, Gloria E O (2017) Substrate-analog binding and electrostatic surfaces of human manganese superoxide dismutase. J Struct Biol 199:68-75
Bonham-Carter, Oliver; Thapa, Ishwor; From, Steven et al. (2017) A study of bias and increasing organismal complexity from their post-translational modifications and reaction site interplays. Brief Bioinform 18:69-84
Donze-Reiner, Teresa; Palmer, Nathan A; Scully, Erin D et al. (2017) Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs. BMC Plant Biol 17:46
Quispe, Cristian F; Esmael, Ahmed; Sonderman, Olivia et al. (2017) Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains. Virology 500:103-113
Gerald, Gary W; Thompson, Moriah M; Levine, Todd D et al. (2017) Interactive effects of leg autotomy and incline on locomotor performance and kinematics of the cellar spider, Pholcus manueli. Ecol Evol 7:6729-6735
Gong, Qiang; Wang, Chao; Zhang, Weiwei et al. (2017) Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data. Sci Rep 7:11301

Showing the most recent 10 out of 322 publications