This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The main goal of this project is to elucidate the functions of the KIN3 protein kinase in Saccharomyces cerevisiae. KIN3 is the S. cerevisiae member of the NIMA family of protein kinases, one of the most poorly understood families of mitotic protein kinases. The family appears to be among the least functionally conserved mitotic regulators, having been implicated in chromosome condensation in fungi and spindle formation in metazoans. The experimental plan includes: (1) The characterization of the effects of deletion of Kin3, a nonessential gene, by fluorescence microscopy, analysis of effects on cell cycle progression, and drug sensitivity tests; (2) Identification of genes that interact with Kin3 by high-throughput synthetic genetic analysis, followed by characterization of the phenotypic effects of double deletions; and (3) Localization of Kin3p in cells at various cell cycle phases. These studies will aid in understanding the functions of the highly variable NIMA protein kinase family to which KIN3 belongs and will provide significant information regarding the roles of the NIMA family of protein kinases in mitotic regulation.
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