Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the model eukaryote Saccharomyces cerevisiae, cell cycle events are coordinated with constitutive functions like energy generation and duplication of protein mass. The latter processes are stimulated by the Rap1p/Gcr1p/Gcr2p complex, which activates transcription of the genes required for rapid growth;this includes glycolytic and ribosomal protein genes, which are among the most heavily transcribed in the cell. In vivo 32P labeling has shown that Rap1p, Gcr1p, and Gcr2p are all phosphoproteins. Mapping the exact sites of phosphorylation in these proteins would represent an important advance in our understanding of the signaling cascade(s) that regulates the Rap1 activation complex. Mass spectrometry (LC-MS/MS) will be used to analyze phosphorylation sites in these factors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016476-10
Application #
8360564
Study Section
Special Emphasis Panel (ZRR1-RI-7 (01))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
10
Fiscal Year
2011
Total Cost
$105,880
Indirect Cost

  Institution

Name
University of Southern Mississippi
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
623335775
City
Hattiesburg
State
MS
Country
United States
Zip Code
39406

  Publications

Amato, Douglas V; Amato, Dahlia N; Blancett, Logan T et al. (2018) A bio-based pro-antimicrobial polymer network via degradable acetal linkages. Acta Biomater 67:196-205
Dutta, Shovan; Celestine, Michael J; Khanal, Supreet et al. (2018) Coordination of different ligands to copper(II) and cobalt(III) metal centers enhances Zika virus and dengue virus loads in both arthropod cells and human keratinocytes. Biochim Biophys Acta Gen Subj 1862:40-50
Budachetri, Khemraj; Kumar, Deepak; Crispell, Gary et al. (2018) The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vector. Microbiome 6:141
Das, Pradipta K; Dean, Dexter N; Fogel, April L et al. (2017) Aqueous RAFT Synthesis of Glycopolymers for Determination of Saccharide Structure and Concentration Effects on Amyloid ? Aggregation. Biomacromolecules 18:3359-3366
Rana, Pratip; Dean, Dexter N; Steen, Edward D et al. (2017) Fatty Acid Concentration and Phase Transitions Modulate A? Aggregation Pathways. Sci Rep 7:10370
Dean, Dexter N; Das, Pradipta K; Rana, Pratip et al. (2017) Strain-specific Fibril Propagation by an A? Dodecamer. Sci Rep 7:40787
Budachetri, Khemraj; Williams, Jaclyn; Mukherjee, Nabanita et al. (2017) The microbiome of neotropical ticks parasitizing on passerine migratory birds. Ticks Tick Borne Dis 8:170-173
Budachetri, K; Kumar, D; Karim, S (2017) Catalase is a determinant of the colonization and transovarial transmission of Rickettsia parkeri in the Gulf Coast tick Amblyomma maculatum. Insect Mol Biol 26:414-419
Budachetri, Khemraj; Crispell, Gary; Karim, Shahid (2017) Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity. Insect Biochem Mol Biol 88:37-47
Bullard, Rebekah L; Williams, Jaclyn; Karim, Shahid (2016) Temporal Gene Expression Analysis and RNA Silencing of Single and Multiple Members of Gene Family in the Lone Star Tick Amblyomma americanum. PLoS One 11:e0147966

Showing the most recent 10 out of 143 publications