This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In recent years the role of glycosylation to proteins has been under increased investigation as the resulting glycoproteins have been shown to influence protein function and activity. Because multiple glycosylation sites can exist and a large number of glycan structures can potentially be associated with each site, the number of possible structural combinations is frequently very large. The possible number of combination increases greatly with the size of the molecular size of the protein. The resolution and identification of glycan structure and the various points of attachment has proven to be very challenging analytical project and has not been adequately addressed. Even the most powerful mass spectrometric techniques cannot frequency resolve isobaric structures in glycan analysis. We propose to investigate the potential of capillary electrophoresis in the analysis of glycoproteins using a group of model glycoproteins. First, we will examine the potential of electrophoretic mobility shifts induced by borate complexation of glycopeptides in trypsin digest of model glycoproteins using capillary electrophoresis with laser induced fluorescence detection. This technique could rapidly identify which peptides are glycosylated. We will also explore the potential of capillary electrophoresis-mass spectrometry in the analysis of complex glycan mix1ures that have been enzymatically released from model glycoproteins using borate-based electrolytes. Both of these related analysis techniques could provide critical information in the structural analysis of medically significant glycoproteins and could develop into powerful tools in routine glycomics analysis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016478-10
Application #
8167538
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2010-04-01
Project End
2011-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
10
Fiscal Year
2010
Total Cost
$33,678
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2018) Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis. Sci Rep 8:7805
Wetherill, Marianna S; Williams, Mary B; Gray, Karen A (2017) SNAP-Based Incentive Programs at Farmers' Markets: Adaptation Considerations for Temporary Assistance for Needy Families (TANF) Recipients. J Nutr Educ Behav 49:743-751.e1
Hannafon, Bethany N; Trigoso, Yvonne D; Calloway, Cameron L et al. (2016) Plasma exosome microRNAs are indicative of breast cancer. Breast Cancer Res 18:90
Wilson, Kevin R; Cannon-Smith, Desiray J; Burke, Benjamin P et al. (2016) Synthesis and structural studies of two pyridine-armed reinforced cyclen chelators and their transition metal complexes. Polyhedron 114:118-127
Trigoso, Yvonne D; Evans, Russell C; Karsten, William E et al. (2016) Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase. PLoS One 11:e0146525
Khandaker, Morshed; Riahinezhad, Shahram; Sultana, Fariha et al. (2016) Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. Int J Nanomedicine 11:585-94
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2016) Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis. Sci Rep 6:27453
Khandaker, Morshed; Meng, Zhaotong (2015) The Effect of Nanoparticles and Alternative Monomer on the Exothermic Temperature of PMMA Bone Cement. Procedia Eng 105:946-952
Guthmiller, Jenna J; Zander, Ryan A; Butler, Noah S (2015) Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice. Methods Mol Biol 1325:19-37
Jones, Donald G; Wilson, Kevin R; Cannon-Smith, Desiray J et al. (2015) Synthesis, structural studies, and oxidation catalysis of the late-first-row-transition-metal complexes of a 2-pyridylmethyl pendant-armed ethylene cross-bridged cyclam. Inorg Chem 54:2221-34

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