Abstract

The overall hypothesis of the proposed research is that the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) plays a regulatory role in the differential expression of the gonadotropin subunit genes that occurs during fetal and early postnatal sexual maturation. We propose that pituitary exposure to PACAP stimulates LHbeta and the common glycoprotein alpha-subunit but, delays the expression of FSHbeta during fetal development, and that a decrease in PACAP exposure results in the monotropic rise in FSH that occurs during the juvenile stage of sexual maturation in the male rat.
The Specific Aims of the present proposal are:
Aim 1. To determine whether PACAP plays a role in the differential expression of the gonadotropin subunit genes during fetal pituitary development.
Aim 2. To determine if pituitary cells from fetal and infant rats are more responsive to PACAP than are cells from juvenile rats.
Aim 3. To determine if the rose in FSHbeta mRNA in immature male rats is related to weaning, and is coincident with a change in PACAP expression in the hypothalamus or pituitary gland.
Aim 4. To explore the developmental effects of PACAP through pituitary specific expression of the potent PACAP antagonist PACAP-(6-38).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR017702-01
Application #
6688040
Study Section
Special Emphasis Panel (ZRR1)
Project Start
2002-09-30
Project End
2007-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Louisville
Department
Type
DUNS #
City
Louisville
State
KY
Country
United States
Zip Code
40292

  Publications

Jin, Jiu-Zhen; Lei, Zhenmin; Lan, Zi-Jian et al. (2018) Inactivation of Fgfr2 gene in mouse secondary palate mesenchymal cells leads to cleft palate. Reprod Toxicol 77:137-142
Liu, Lingyun; He, Yan; Guo, Kaimin et al. (2017) Ggnbp2-Null Mutation in Mice Leads to Male Infertility due to a Defect at the Spermiogenesis Stage. Am J Pathol 187:2508-2519
Liu, Xiao; Tang, Luosheng; Liu, Yongqing (2017) Mouse Müller Cell Isolation and Culture. Bio Protoc 7:
Mukhopadhyay, Partha; Seelan, Ratnam S; Rezzoug, Francine et al. (2017) Determinants of orofacial clefting I: Effects of 5-Aza-2'-deoxycytidine on cellular processes and gene expression during development of the first branchial arch. Reprod Toxicol 67:85-99
Li, Shengqiang; Moore, Andrew K; Zhu, Jia et al. (2016) Ggnbp2 Is Essential for Pregnancy Success via Regulation of Mouse Trophoblast Stem Cell Proliferation and Differentiation. Biol Reprod 94:41
Lan, Zi-Jian; Hu, YunHui; Zhang, Sheng et al. (2016) GGNBP2 acts as a tumor suppressor by inhibiting estrogen receptor ? activity in breast cancer cells. Breast Cancer Res Treat 158:263-76
Neal, Rachel E; Chen, Jing; Webb, Cindy et al. (2016) Developmental cigarette smoke exposure II: Hepatic proteome profiles in 6 month old adult offspring. Reprod Toxicol 65:414-424
Neal, Rachel E; Jagadapillai, Rekha; Chen, Jing et al. (2016) Developmental cigarette smoke exposure II: Kidney proteome profile alterations in 6 month old adult offspring. Reprod Toxicol 65:425-435
Warner, Dennis R; Smith, Scott C; Smolenkova, Irina A et al. (2016) Inhibition of p300 histone acetyltransferase activity in palate mesenchyme cells attenuates Wnt signaling via aberrant E-cadherin expression. Exp Cell Res 342:32-8
Neal, Rachel E; Jagadapillai, Rekha; Chen, Jing et al. (2016) Developmental cigarette smoke exposure II: Hippocampus proteome and metabolome profiles in adult offspring. Reprod Toxicol 65:436-447

Showing the most recent 10 out of 114 publications