This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The entire spectrum of expressed proteins expressed in a cell at a given time is described as the proteome, as distinguished from the genome, the DNA that codes for proteins and other gene products. A single gene can express many different forms of proteins, and post-transcriptional modification of proteins can result in further variety such that the proteome is distinct from, and can be quite different in character and makeup from the genome or even the mRNA expressed genome. Proteomics involves the qualitative and quantitative analysis of the expressed proteome in biological systems and required highly specialized training and instrumentation to accomplish Thus, a proteomics core is an important component of a modern biomedical research enterprise. Proteomics is a major component of four of the five initial research projects of our COBRE program, as well as a component of the research of the senior mentors of this program. Our goal is to develop through the COBRE grant a state-of-the-art Proteomics Facility Core at Dartmouth with a complete series of proteomics-related services. This includes recruitment of a faculty proteomics expert to Dartmouth, renovation of the nascent Molecular Biology & Proteomics Core facility space at Dartmouth, training of professional staff for the core, and acquisition of state-of-the-art instrumentation. In Year Three we successfully recruited a proteomics expert, began renovations of the core facility, began acquisition of instruments, and began a comprehensive training and methodology review of the core's operations and staff. We have continued this process in the current year with great success as described below.
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