Abstract

The Animal/Transgenic Core will provide vertebrate animals and related services to Buck Institute investigators and to investigators at other institutions. General animal services will include training scientific staff in animal care and in vivo experimental procedures, providing basic vertebrate animal husbandry in an AAALAC-accredited facility, serological testing of animals for pathogens, veterinary care and consultation, necropsy services, quarantine procedures, euthanasia training and services, selected lACUC-approved surgical and nonsurgical procedures, tissue collection and storage, and transportation of animals, tissues and cells to outside investigators. Transgenic services will include production of transgenic and gene-knockout mice, breeding of genetically modified mice, genotyping, anatomical characterization of transgene expression (in collaboration with the Imaging Core), cryopreservation of transgenic mouse lines, and periodic analysis of mouse lines to ensure genetic stability.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Center Core Grants (P30)
Project #
5P30AG025708-04
Application #
7653606
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
4
Fiscal Year
2008
Total Cost
$114,963
Indirect Cost

  Institution

Name
Buck Institute for Age Research
Department
Type
DUNS #
786502351
City
Novato
State
CA
Country
United States
Zip Code
94945

  Publications

Guyenet, Stephan J; Mookerjee, Shona S; Lin, Amy et al. (2015) Proteolytic cleavage of ataxin-7 promotes SCA7 retinal degeneration and neurological dysfunction. Hum Mol Genet 24:3908-17
Chen, Di; Li, Patrick Wai-Lun; Goldstein, Benjamin A et al. (2013) Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep 5:1600-10
Flynn, James M; Melov, Simon (2013) SOD2 in mitochondrial dysfunction and neurodegeneration. Free Radic Biol Med 62:4-12
Miller, John P; Yates, Bridget E; Al-Ramahi, Ismael et al. (2012) A genome-scale RNA-interference screen identifies RRAS signaling as a pathologic feature of Huntington's disease. PLoS Genet 8:e1003042
Flynn, James M; Czerwieniec, Gregg A; Choi, Sung W et al. (2012) Proteogenomics of synaptosomal mitochondrial oxidative stress. Free Radic Biol Med 53:1048-60
Goehring, Isabel; Gerencser, Akos A; Schmidt, Sara et al. (2012) Plasma membrane potential oscillations in insulin secreting Ins-1 832/13 cells do not require glycolysis and are not initiated by fluctuations in mitochondrial bioenergetics. J Biol Chem 287:15706-17
Katewa, Subhash D; Demontis, Fabio; Kolipinski, Marysia et al. (2012) Intramyocellular fatty-acid metabolism plays a critical role in mediating responses to dietary restriction in Drosophila melanogaster. Cell Metab 16:97-103
Mookerjee, Shona A; Brand, Martin D (2011) Characteristics of the turnover of uncoupling protein 3 by the ubiquitin proteasome system in isolated mitochondria. Biochim Biophys Acta 1807:1474-81
Powolny, Anna A; Singh, Shivendra V; Melov, Simon et al. (2011) The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation. Exp Gerontol 46:441-52
Flynn, James M; Choi, Sung W; Day, Nicholas U et al. (2011) Impaired spare respiratory capacity in cortical synaptosomes from Sod2 null mice. Free Radic Biol Med 50:866-73

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