Core - Immunology

Zolla-Pazner, Susan B.

Abstract

The Immunology Core provides basic HIV-related immunologic tests to researchers in the NYU CFAR. It is directed by Dr. Susan Zolla-Pazner, whose laboratory has been performing immunologic assays since the beginning of the AIDS epidemic in 1981. This core laboratory has grown to meet the needs of various researcher within CFAR. For example, HIV testing by ELISA and Western blot is offered as is testing of indeterminant specimens by radioimmunoprecipitation, upon request. p24 antigen testing is also offered; while this is not a component of standard work-ups, it has found usefulness in projects that require the identification of patients with significant viremia. Other immunologic assays include quantitation of beta-2 microglobulin and various cytokines which may be important in the immune regulation of HIV infection. Flowcytometry analysis is offered on routine blood specimens as well as on bronchoalveolar lavage specimens and research specimens that require special stains and analytic procedures. Several new assays are contemplated in the new CFAR. These include proliferative assays for lymphocyte activity against various antigens, assays for natural killer cell activity, and quantitation of antibody activity against various pathogens (hepatitis C, HTLV-I or II, etc.). In addition, B-cell precursor frequency assays will be offered. This laboratory is already fully equipped. Costs for supplies will be recovered by a charge-back system which is already in place.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Center Core Grants (P30)
• Project #: 5P30AI027742-09
• Application #: 6234907
• Project Start: 1997-07-01
• Project End: 1998-06-30
• Budget Start: 1996-10-01
• Budget End: 1997-09-30
• Support Year: 9
• Fiscal Year: 1997

Institution

• Name: New York University
• City: New York
• State: NY
• Country: United States
• Zip Code: 10016

Publications

Gornalusse, German G; Mummidi, Srinivas; Gaitan, Alvaro A et al. (2015) Epigenetic mechanisms, T-cell activation, and CCR5 genetics interact to regulate T-cell expression of CCR5, the major HIV-1 coreceptor. Proc Natl Acad Sci U S A 112:E4762-71

Miller, Elizabeth A; Gopal, Ramya; Valdes, Vanessa et al. (2015) Soluble CD40 ligand contributes to dendritic cell-mediated T-cell dysfunction in HIV-1 infection. AIDS 29:1287-96

Weiden, Michael D; Hoshino, Satomi; Levy, David N et al. (2014) Adenosine deaminase acting on RNA-1 (ADAR1) inhibits HIV-1 replication in human alveolar macrophages. PLoS One 9:e108476

Ryndak, Michelle B; Singh, Krishna K; Peng, Zhengyu et al. (2014) Transcriptional profiling of Mycobacterium tuberculosis replicating ex vivo in blood from HIV- and HIV+ subjects. PLoS One 9:e94939

Phelan, Joan A; Abrams, William R; Norman, Robert G et al. (2014) Design aspects of a case-control clinical investigation of the effect of HIV on oral and gastrointestinal soluble innate factors and microbes. PLoS One 9:e112901

Sivapalasingam, Sumathi; Mendillo, Megan; Ahmed, Aabid et al. (2014) The importance of caregivers in the outcome of pediatric HIV management, Mombasa, Kenya. AIDS Care 26:425-33

Weiser, Keren; Barton, Meredith; Gershoony, Dafna et al. (2013) HIV's Nef interacts with ?-catenin of the Wnt signaling pathway in HEK293 cells. PLoS One 8:e77865

O'Brien, Meagan; Montenont, Emilie; Hu, Liang et al. (2013) Aspirin attenuates platelet activation and immune activation in HIV-1-infected subjects on antiretroviral therapy: a pilot study. J Acquir Immune Defic Syndr 63:280-8

Rich, Josiah D; DiClemente, Ralph; Levy, Judith et al. (2013) Correctional facilities as partners in reducing HIV disparities. J Acquir Immune Defic Syndr 63 Suppl 1:S49-53

Alvarez, Yelina; Tuen, Michael; Nàdas, Arthur et al. (2012) In vitro restoration of Th17 response during HIV infection with an antiretroviral drug and Th17 differentiation cytokines. AIDS Res Hum Retroviruses 28:823-34