The Molecular Biology Core has been in operation for the past twelve years and has provided state-ofthe-art methodologies for numerous molecular biological techniques and recombinant protein expression andpurification. The major goals of the Molecular Biology Core remain:1. To collect and maintain suitable plasmid vectors and host for expression of HIV/SIV and relatedrecombinant proteins2. To provide a service for construction of recombinant plasmids and high level expression of HIV/SIVand related proteins, including HLA tetramers for immunological analysis.3. To provide a service for purification of HIV/SIV and related proteins.4. To establish an onsite repository for plasmids with HIV/SIV genes as well as recombinant HIV/SIVproteins.5. To provide a service for construction of infectious HIVISIV proviral clones containing definedmutations in genes of interest.6. To train personnel to assist in the various phases of plasmid construction and expression/purificationof recombinant proteins for the purpose of facilitating technology transfer.The Molecular Biology Core has assisted, on average, over 25 CFAR members per year during the lastfunding period. The Core has averaged over $40,000 per year in chargebacks over the last funding period($180,000 total). The Molecular Biology Core has provided critical reagents and expertise to UAB CFARmembers that have funded individual R01 grants, UAB's National Corporative Vaccine Development Groupand a multi-investigator funded program project to elucidate features of the HIV-1 reverse transcription. Inthe next funding period, the Core will expand its efforts to provide critical reagents for the international CFARtransmission and vaccine initiatives. Support of this facility is thus instrumental for the continued success ofnumerous investigators working in different areas of HIV pathogenesis, vaccine and therapeuticdevelopment currently conducted at UAB.
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