Abstract

The utilization of cell culture systems for propagation of human or simian immunodeficiency viruses constitutes a necessary element in a wide variety of AIDS-related projects. The goal of this core, consisting of a staffed biohazard containment facility, is to support work with infectious materials under safe conditions. The present facility includes 10 individual fully equipped workrooms, ultracentrifuges, fluorescent microscope an autoclaves. The facility is currently utilized by 49 investigators. A Containment Suite Manager and a full-time technician contribute to the operation and maintenance of the Core as well as to the scientific services offered. The Biohazard Containment Facility and staff allow: 1) Training of research personnel in safe handling of infectious materials. 2) The establishment of working conditions in which scientific progress can be expedited without sacrificing worker safety. 3) Technical Support in the preparation/propagation of virus stocks, characterization of the phenotype of virus variants, and testing the toxicity and efficacy of potential antiviral agents. 4) Facilitation of collaborative interactions between investigators experienced in the propagation of primate immunodeficiency viruses and other investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI028691-10
Application #
6099441
Study Section
Project Start
1998-08-01
Project End
1999-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
10
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Liu, Songtao; Fleysher, Roman; Fleysher, Lazar et al. (2010) Brain metabolites B1-corrected proton T1 mapping in the rhesus macaque at 3 T. Magn Reson Med 63:865-71
Liu, Songtao; Gonen, Oded; Fleysher, Roman et al. (2009) Metabolite proton T(2) mapping in the healthy rhesus macaque brain at 3 T. Magn Reson Med 62:1292-9
Ratai, Eva-Maria; Pilkenton, Sarah J; Greco, Jane B et al. (2009) In vivo proton magnetic resonance spectroscopy reveals region specific metabolic responses to SIV infection in the macaque brain. BMC Neurosci 10:63
Gonen, Oded; Liu, Songtao; Goelman, Gadi et al. (2008) Proton MR spectroscopic imaging of rhesus macaque brain in vivo at 7T. Magn Reson Med 59:692-9
Ratai, Eva-Maria; Hancu, Ileana; Blezek, Daniel J et al. (2008) Automatic repositioning of MRSI voxels in longitudinal studies: impact on reproducibility of metabolite concentration measurements. J Magn Reson Imaging 27:1188-93
Liu, Songtao; Gonen, Oded; Fleysher, Lazar et al. (2008) Regional metabolite T2 in the healthy rhesus macaque brain at 7T. Magn Reson Med 59:1165-9
Li, Xing; Gold, Bert; O'hUigin, Colm et al. (2007) Unique features of TRIM5alpha among closely related human TRIM family members. Virology 360:419-33
Javanbakht, Hassan; An, Ping; Gold, Bert et al. (2006) Effects of human TRIM5alpha polymorphisms on antiretroviral function and susceptibility to human immunodeficiency virus infection. Virology 354:15-27
Diaz-Griffero, Felipe; Li, Xing; Javanbakht, Hassan et al. (2006) Rapid turnover and polyubiquitylation of the retroviral restriction factor TRIM5. Virology 349:300-15
Gonzalez, R Gilberto; Greco, Jane B; He, Julian et al. (2006) New insights into the neuroimmunity of SIV infection by magnetic resonance spectroscopy. J Neuroimmune Pharmacol 1:152-9

Showing the most recent 10 out of 205 publications