The overall purpose of the Molecular Biology Core (Core C) is to provide efficient shared access to basic molecular biology services for investigators for need to apply a variety of molecular techniques to problems involving HIV replication, pathogenesis, therapy, and immunoprophylaxis of HIV infection, with particular emphasis on support of developmental projects. Specific objectives of Core C include: [1] To provide small and large- scale, cost efficient DNA sequencing for CFAR laboratories, [2] To provide accurate message analysis services to core members, [3] To manage a plasmid library, providing large scale preparation of full-length HIV-1 and HIV-2 viral clones, and [4] To manage a PCR primer library for the efficient distribution of primers shared among investigators. Ongoing Molecular Biology Core support of UCSD research efforts clearly illustrates its need and usefulness. Large sequencing efforts supported include sequencing of Coccididiomycosis inmitis, Aspergillus fumigatus, and Toxoplasma gondii, envelope fragments from pediatric and adult CNS sources ongoing analysis pol mutations induced by treatment with protease inhibitors, and analysis of sequence variation in an HIV-2 M. nemestrina vaccine model. Such efforts are crucial to our understanding of viral resistance, and the meaning of viral variation. The recent introduction of quantitative RT PCR assays for chemokine receptor and beta-chemokine messages by Core C has supported research on HIV induced apoptosis, differential co-receptor usage by HIV strains, the role of co-receptors in nef action, and the use of chemokine receptors by other lentiviruses. The Molecular Biology Core plasmid library has facilitated early work on DNA vaccines for HIV, investigation of nef enhancement of infectivity, determination of HIV genes which contribute to apoptosis, work on bacterial vectors for AIDS vaccines. Primers designed by the core have proven valuable to the construction of new and very useful reporter cell lines bearing CCR5 receptors. The proposed Molecular Biology Core (Core C) represents an extension of a model service core which has been thoroughly tested and proven to be a productive, cost-efficient and useful core resource in the existing UCSD Center for AIDS Research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
2P30AI036214-06
Application #
6268170
Study Section
Project Start
1998-09-01
Project End
1999-02-28
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Garfein, Richard S; Liu, Lin; Cuevas-Mota, Jazmine et al. (2018) Tuberculosis Treatment Monitoring by Video Directly Observed Therapy in 5 Health Districts, California, USA. Emerg Infect Dis 24:1806-1815
Heldt, Sven; Prattes, Juergen; Eigl, Susanne et al. (2018) Diagnosis of invasive aspergillosis in hematological malignancy patients: Performance of cytokines, Asp LFD, and Aspergillus PCR in same day blood and bronchoalveolar lavage samples. J Infect 77:235-241
Jenks, Jeffrey Daniel; Hoenigl, Martin (2018) CD4:CD8 ratio and CD8+ cell count for prognosticating mortality in HIV-infected patients on antiretroviral therapy. J Lab Precis Med 3:
Canan, Chelsea E; Chander, Geetanjali; Monroe, Anne K et al. (2018) High-Risk Prescription Opioid Use Among People Living With HIV. J Acquir Immune Defic Syndr 78:283-290
Jiang, Wei; Luo, Zhenwu; Martin, Lisa et al. (2018) Drug Use is Associated with Anti-CD4 IgG-mediated CD4+ T Cell Death and Poor CD4+ T Cell Recovery in Viral-suppressive HIV-infected Individuals Under Antiretroviral Therapy. Curr HIV Res 16:143-150
White, Cory H; Beliakova-Bethell, Nadejda; Lada, Steven M et al. (2018) Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment. Front Immunol 9:603
Gianella, Sara; Marconi, Vincent C; Berzins, Baiba et al. (2018) Genital HIV-1 Shedding With Dolutegravir (DTG) Plus Lamivudine (3TC) Dual Therapy. J Acquir Immune Defic Syndr 79:e112-e114
Innes, Steve; Patel, Kunjal (2018) Noncommunicable diseases in adolescents with perinatally acquired HIV-1 infection in high-income and low-income settings. Curr Opin HIV AIDS 13:187-195
Morales, Mario; Rafful, Claudia; Gaines, Tommi L et al. (2018) Factors associated with extrajudicial arrest for syringe possession: results of a department-wide survey of municipal police in Tijuana, Mexico. BMC Int Health Hum Rights 18:36
Rhodes, Tim (2018) The becoming of methadone in Kenya: How an intervention's implementation constitutes recovery potential. Soc Sci Med 201:71-79

Showing the most recent 10 out of 921 publications