Abstract

The group A streptococcus (Streptococcus pyogenes, GAS) is unsurpassed among bacterial pathogens in its ability to cause a variety of skin infections ranging from the self-limiting impetigo to fulminant soft issue destruction and necrotizing fasciitis. GAS pathogenes is a multi- factorial process,, involving numerous virulence components are involved in the specific response of the bacterial to different environments encountered within the host. However, we know very little about when these different virulence products are produced during infection. The overall aim of this pilot proposal will be to establish an in vivo receptor system based on the Green Fluorescent Protein (GFP) to assay GAS virulence gene expression during skin disease in an animal model.
The specific aims of the project will be as follows: 1. To construct GAS vectors for transcriptional fusions to gfp and test their capabilities in vitro. 2. To establish in vivo conditions for assaying GFP expression in a mouse model for streptococcal invasive skin disease. 3. To assay in vivo expression of known virulence genes in a mouse model of streptococcal invasive skin disease. These studies will hopefully lead to the establishment of a useful model system for directly examining the host/pathogen interplay between GAS and human host during invasive skin infections.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
2P30AR041940-10
Application #
6459525
Study Section
Special Emphasis Panel (ZAR1)
Project Start
1997-06-01
Project End
2006-05-31
Budget Start
Budget End
Support Year
10
Fiscal Year
2001
Total Cost
$55,151
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Acharya, Asha; Baek, Seung Tae; Banfi, Serena et al. (2011) Efficient inducible Cre-mediated recombination in Tcf21 cell lineages in the heart and kidney. Genesis 49:870-7
Straud, Sarah; Zubovych, Iryna; De Brabander, Jef K et al. (2010) Inhibition of iron uptake is responsible for differential sensitivity to V-ATPase inhibitors in several cancer cell lines. PLoS One 5:e11629
Chapman, Shelby L; Sicot, F-X; Davis, Elaine C et al. (2010) Fibulin-2 and fibulin-5 cooperatively function to form the internal elastic lamina and protect from vascular injury. Arterioscler Thromb Vasc Biol 30:68-74
Yanagisawa, Hiromi; Davis, Elaine C (2010) Unraveling the mechanism of elastic fiber assembly: The roles of short fibulins. Int J Biochem Cell Biol 42:1084-93
Choi, Jiwon; Bergdahl, Andreas; Zheng, Qian et al. (2009) Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. Matrix Biol 28:211-20
Jaeckle Santos, Lane J; Xing, Chao; Barnes, Robert B et al. (2008) Refined mapping of X-linked reticulate pigmentary disorder and sequencing of candidate genes. Hum Genet 123:469-76
Takayama, Yoshiharu; May, Petra; Anderson, Richard G W et al. (2005) Low density lipoprotein receptor-related protein 1 (LRP1) controls endocytosis and c-CBL-mediated ubiquitination of the platelet-derived growth factor receptor beta (PDGFR beta). J Biol Chem 280:18504-10
Wang, Huixia; He, Xuming; Band, Mark et al. (2005) A study of inter-lab and inter-platform agreement of DNA microarray data. BMC Genomics 6:71
Sinha, Suwan K; Zachariah, Sunny; Quinones, Herson I et al. (2002) Role of TRAF3 and -6 in the activation of the NF-kappa B and JNK pathways by X-linked ectodermal dysplasia receptor. J Biol Chem 277:44953-61
Sellati, T J; Waldrop, S L; Salazar, J C et al. (2001) The cutaneous response in humans to Treponema pallidum lipoprotein analogues involves cellular elements of both innate and adaptive immunity. J Immunol 166:4131-40

Showing the most recent 10 out of 56 publications