The purposes of the YSDRCC Tissue Acquisition and Analysis Core (TAAC) are to stimulate and facilitatecutaneous research by providing highly efficient and cost-effective services, facilities, and expert assistancein the acquisition and analysis of samples of normal and diseased skin. The TAAC will facilitate thepreparation and analysis of tissue sections derived from experimental tissues provided by investigators, orfrom archival tissue accessible to the TAAC facility. Sample sources in hand consist of ~500,000 biopsyspecimen archive of diseased skin and serum samples that were submitted to the Yale DermatopathologyService, and that are cross-referenced by diagnosis in a relational database. Tissue sections and nucleicacids extracted from multiple cases of common and rare diseases can be provided to researchers from thistissue bank, in accordance with Yale Human Investigation Committee guidelines and HIPAA regulations.The expertise and volume of samples associated with this unique combination will allow the proficientdelivery of the following specific services, including, but not limited to: (1) preparation of unstained tissuesections and histochemical stained sections; (2) immunohistochemical and immunofluorescence staining oftissue sections; (3) dual-color flow cytometric analysis and sample preparation, including the isolation andstaining of fresh leukocytes and cultured cells, as well as cells isolated from fresh epidermis; (4) expertassistance in the morphologic analysis of tissue sections, including histochemical and immunohistochemicalstudies; (5) training of investigators in a variety of techniques used in the routine processing of skin, theperformance of special staining techniques such as immunoperoxidase and immunofluorescence; (6)access to fresh-frozen banked skin and related tissues through active collaboration between this Core andseveral modules of the Critical Technologies unit of the Department of Pathology; (7) preparation of tissuefor and performance of tissue microarrays; and 8) training in the use of, and access to new, state-of-the artimaging technologies, including laser capture dissection microscopy and intravital (two-photon) microscopy.Funding of this Core will encourage investigation of skin diseases by providing appropriate samples toresearchers, including those whose primary area of interest is not the skin, and by providing economies ofscale and the highest levels of expertise to investigators.
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