Abstract

The morphology and cell analysis core will serve as a resource for Harvard Skin Disease Research Center investigators to characterize skin-related disorders by various techniques, including: 1) routine histopathology; 2) immunohistochemistry, utilizing both frozen and paraffin-embedded material for both immunoperoxidase and immunofluorescence analysis; 3) in situ hybridization for both frozen and paraffin-embedded tissue sections, utilizing both radioisotope-labeled and biotinylated probes; 4) electron microscopy of epon-embedded cells and tissue sections, and frozen cryosectioned tissue; 5) Fluorescence based flow cytometry and microscopy for quantitative and qualitative analysis of cell surface and intracellular molecules; and 6) photographic or computer-based recording of images derived from 1-5. This core will contain the necessary equipment, reagents, and technical expertise to conduct sophisticated research using the methods described above. Furthermore, personnel in the MCA core will be available to advise investigators on research design and also to assist in the interpretation of results.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR042689-03
Application #
5206343
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Tian, Tian; Jin, Michelle Qiushuang; Dubin, Krista et al. (2017) IL-1R Type 1-Deficient Mice Demonstrate an Impaired Host Immune Response against Cutaneous Vaccinia Virus Infection. J Immunol 198:4341-4351
Pan, Youdong; Tian, Tian; Park, Chang Ook et al. (2017) Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature 543:252-256
Volpicelli, Elgida R; Lezcano, Cecilia; Zhan, Qian et al. (2014) The multidrug-resistance transporter ABCB5 is expressed in human placenta. Int J Gynecol Pathol 33:45-51
Guenova, Emmanuella; Watanabe, Rei; Teague, Jessica E et al. (2013) TH2 cytokines from malignant cells suppress TH1 responses and enforce a global TH2 bias in leukemic cutaneous T-cell lymphoma. Clin Cancer Res 19:3755-63
Majewska-Szczepanik, Monika; Paust, Silke; von Andrian, Ulrich H et al. (2013) Natural killer cell-mediated contact sensitivity develops rapidly and depends on interferon-?, interferon-? and interleukin-12. Immunology 140:98-110
Zadran, Sohila; McMickle, Robert; Shackelford, David et al. (2013) Monitoring extra-vascular migratory metastasis (EVMM) of migrating cancer cells using an in vitro co-culture system. Protoc exch 2013:
Burkhardt, Ute E; Hainz, Ursula; Stevenson, Kristen et al. (2013) Autologous CLL cell vaccination early after transplant induces leukemia-specific T cells. J Clin Invest 123:3756-65
Dowlatshahi, Mitra; Huang, Victor; Gehad, Ahmed E et al. (2013) Tumor-specific T cells in human Merkel cell carcinomas: a possible role for Tregs and T-cell exhaustion in reducing T-cell responses. J Invest Dermatol 133:1879-89
Seneschal, Julien; Clark, Rachael A; Gehad, Ahmed et al. (2012) Human epidermal Langerhans cells maintain immune homeostasis in skin by activating skin resident regulatory T cells. Immunity 36:873-84
Girouard, Sasha D; Laga, Alvaro C; Mihm, Martin C et al. (2012) SOX2 contributes to melanoma cell invasion. Lab Invest 92:362-70

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