The key features of the Molecular Biology Core of the Skin Disease Research Center are itscomprehensive range of services designed to support the needs of both proposed and future SDRCmembers, and its objective of training young investigators in the area of its expertise. This Coreprovides assistance in three essential areas of molecular biology central to virtually any studiesranging from the investigation of the genetic basis of human disease to cell biology experiments.These include 1) Disease-gene regulation; 2) In Situ labeling of genetic alterations; and 3) Real-Time PCR, automated sequencing and cloning of DNA constructs. The core will assist centerinvestigators in identifying the epigenetic mechanisms of a particular skin disease by promoter andmutagenesis studies and siRNA knockdown models. The Core will assist investigators in automatedDNA sequence analysis to identify mutations implicated in skin disease pathogenesis andsubsequent histological detection of target cells harboring these genetic mutations. The Core willalso assist investigators with the design and optimization of Real-time PCR analysis for candidatedisease-genes and developing a plan for ascertaining their functional consequence, and the way inwhich it elicits a clinical phenotype. The Core will also provide instrumentation, general resourcesand technical expertise in the use of automated DNA sequencing and DNA cloning. By offeringthese routinely used services, the Molecular Biology Core will interact closely with the other twocores. Further, the expertise offered in this Core will be almost universally utilized and provide avaluable service to most, if not all, of the Pilot and Feasibility studies, as well as other investigatorswithin the Department.
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